硫酸钙/聚氨基酸复合三联抗痨药人工缓释材料的制备及物理性能测定  

The preparation of new calcium sulfate/amino acid polymer artificial bone carrying 3 anti-TB drugs and evaluation of physical properties

作　　者：刘海涛[1] 施建党[2] 王骞[3] 王自立[2] 耿广起[2] 杨宗强[2] 王洁[4] 

机构地区：[1]宁夏医科大学总医院创伤骨科,银川市750004 [2]宁夏医科大学总医院脊柱骨科,银川市750004 [3]美国南佛罗里达大学药学院,坦帕33612 [4]宁夏医科大学科学技术实验中心,银川市750004

出　　处：《中国矫形外科杂志》2015年第21期1984-1988,共5页Orthopedic Journal of China

基　　金：国家自然科学基金项目(代码81060149);宁夏自然科学基金项目(代码NZ10117)

摘　　要：[目的]通过压缩强度的测定和扫描电镜的观察,评价本研究制备的载三联抗痨药HRZ硫酸钙/聚氨基酸人工缓释材料的物理性能,从而制定构成缓释材料各成分的优化配比方案。[方法]实验组分为A1、A2、A3、A4、A5五个亚组,避光环境下分别根据五种不同比例的配比方案,称取适量硫酸钙/聚氨基酸、异烟肼(INH,H)、利福平(RFP,R)、吡嗪酰胺(PZA,Z)制备成直径9 mm、高6 mm圆柱状载药人工缓释材料,每一亚组制备8块,干燥。对照组B组为不含任何药物的人工缓释材料,以同法制备8块。将制备好的人工缓释材料在电子力学万能材料试验机上测定压缩强度;并在扫描电子显微镜下观察显微结构。[结果](1)实验组各亚组药占比重量A19.09%、A212.28%、A313.79%、A416.67%、A528.57%时,压缩强度分别依次为(11.63±0.18)MPa、(10.74±0.25)MPa、(10.11±0.24)MPa、(3.48±0.22)MPa、(1.18±0.18)MPa;对照组不含药物,压缩强度为(18.62±0.16)MPa;6组数据分别进行组间比较,存在差异,具有统计学意义(F=7 141.068,P=0.000);(2)硫酸钙/聚氨基酸人工缓释材料晶体之间存在大量的空隙,为承载药物提供了丰富的空间。[结论]载三联抗痨药HRZ硫酸钙/聚氨基酸人工缓释材料随着抗痨药物药占比的增加,压缩强度逐渐降低;但在一定药物含量范围内,人工材料的强度变化并不明显;硫酸钙晶体间的空隙是该人工材料承载抗痨药物的空间。[Objective] To evaluate physical properties of 3anti- TB drugs loaded carrier of calcium sulfate / amino acid polymer prepared in this study by compressive strength testing and scanning electron microscopy,for optimizing the compounding ratio. [Methods] Expermental treatment was divided into five subgroups： A1,A2,A3,A4 and A5. The corresponding 5 carriers were made,in a shape of cylinder 6 mm long and 9 mm in diameter,from calcium sulfate / poly amino acid,isoniazid（ INH,H）,rifampicin（ RFP,R） motrazubanude（ PZA,Z） in 5different proportions in dark environment,with 8 samples in each subgroup. In addition,no drug- loaded carrier made by the same method was used as control group. The samples were tested with the electronic universal testing machine for compressive strength,and observed with scanning eiectron microscope. [Results] As drugs accounting for 9. 09% in A1,12. 28% in A2,13. 79% in A3,16. 67% in A4 and 28. 57% in A5,compressive strength was（ 11. 63 ± 0. 16） MPa,（ 10. 11 ± 0. 24） MPa,（ 3. 48 ± 0. 22） MPa and（ 1. 18 ± 0. 18） MPa respectively. By contrast,it was（ 1862 ± 0. 16） MPa in the control group. There were statistical differences among the goups（ F = 7 141. 07,P 〈0. 001）. [Conclusion] As content of anti- TB drugs increase,compressive strength of the triple anti- TB drugs loaded carrier decreases. However,in a certain range of drug content increase,strength of the carrier does not change significantly. The gaps of calcium sulfate crystals are filled with anti- tuberculosis drugs.

关 键 词：抗痨药 硫酸钙/聚氨基酸人工材料 抗压缩强度 扫描电镜 

分 类 号：R318.08[医药卫生—生物医学工程]