兔和猴眼表碱烧伤经人羊膜治疗性移植后的临床表现和病理变化观察  

Clinical manifestations and pathological changes of ocular surface alkali injury in rabbits and monkeys after human amniotic membrane transplantation

作　　者：张和宁 张敏[1] 黄勉 黎韦华[1] 王文聪[1] 叶志民[1] 李志权[1] 王智崇[1] 黄冰[1] 

机构地区：[1]中山大学中山眼科中心眼科学国家重点实验室,广州510060 [2]广州市动物园,510070

出　　处：《临床眼科杂志》2015年第5期452-455,共4页Journal of Clinical Ophthalmology

基　　金：广东省科技项目(No.2013B040200020);眼科学国家重点实验室自助研究课题(2012P105)

摘　　要：目的观察兔和猴眼表碱烧伤经人羊膜移植治疗后的临床和病理变化。方法选取6只新西兰兔和3只猕猴构建眼表碱烧伤模型,采用人羊膜移植治疗,于术后第1、2、4、8和12周观察术眼的临床表现。术后12周处死动物,取角膜、角膜缘及巩膜区部分组织,行HE染色。结果经人羊膜移植治疗后,兔角膜碱烧伤后产生较强烈的并发症如角膜结膜化、混浊、新生血管增生等反应;猴角膜碱烧伤后的症状则要轻微的多。结论兔和猴眼表碱烧伤经人羊膜治疗性移植后表现出不同的临床和病理变化,兔子可能更适合眼表碱烧伤模型。Objective To investigate clinical and pathological features of ocular surface alkali injury in rabbits and monkeys after human amniotic membrane transplantation. Methods Six healthy New Zealand rabbits and 3 healthy rhesus monkeys were selected for the model of ocular surface alkali injury. The clinical features of treatment were observed at 1st,2nd,4th,8th,and 12 th weeks after treatment. Animals were sacrificed at 12 th week and the cornea,limbus and sclera tissues were collected and HE stained. Results After treatment of transplantation,the rabbits showed stronger complications such as corneal conjunctiva,turbidity,and neovascularization compared with those in monkeys. Conclusion The rabbits and monkeys showed different clinical and pathological features of ocular surface alkali injury,and rabbits may be more suitable for the model of ocular surface alkali injury.

关 键 词：眼表 碱烧伤 羊膜 兔子 猴 

分 类 号：R779.6[医药卫生—眼科]