北沙参内生真菌的抑菌活性与分类研究  

Study on antimicrobial activity and classification of endophytic fungi from Glehnia littoralis

作　　者：侯晓强[1,2] 任秀艳[1,2] 付亚娟[1,2] 王浩[1] 左秀丽[1] 吕寒聪 张志丽[1] 

机构地区：[1]廊坊师范学院生命科学学院,河北廊坊065000 [2]河北省高校食药用菌资源开发应用技术研发中心,河北廊坊065000

出　　处：《中草药》2015年第19期2932-2936,共5页Chinese Traditional and Herbal Drugs

基　　金：河北省高等学校科学技术研究青年基金项目(2011108;QN2014129);廊坊师范学院微生物学重点学科项目(2015001)

摘　　要：目的研究北沙参内生真菌的抗菌活性,鉴定活性真菌的分类地位。方法采用植物组织分离法从北沙参基原植物珊瑚菜Glehnia littoralis中分离内生真菌。以大肠杆菌、金黄色葡萄球菌和白色假丝酵母为指示菌株,采用牛津杯法研究北沙参内生真菌的抑菌活性,对具有抑菌活性的内生真菌进行分子鉴定。结果有4株内生真菌对大肠杆菌具有抑制作用,内生真菌发酵浓缩液抑菌圈直径与4 U/mL硫酸庆大霉素抑菌圈直径的比值(d/D)最大可达1.07;有15株内生真菌对金黄色葡萄球菌具有抑制作用,d/D值最大为0.65;有3株内生真菌对白色假丝酵母具有抑制作用,内生真菌发酵浓缩液与0.2 mg/mL氟康唑的d/D值最大可达1.27。19株具有抑菌活性的内生真菌归于4个目,5个科,7个属,8个种。结论北沙参植物中存在多种抑菌活性的内生真菌,对于北沙参内生真菌资源的开发及药材道地性的评价具有一定的指导意义。Objective To screen the endophytic fungi with antimicrobial activity from Glehnia littoralis and identify the taxonomy of these fungi. Methods The endophytic fungi were isolated from G. littoralis by method of tissue isolation. The oxford-cup test was used in screening the endophytic fungi with antimicrobial activity for Escherichia coli, Staphylococcus aureus, and Candida albicans respectively. And the r DNA ITS1-5.8S-ITS2 sequences were applied for the taxonomy of endophytic fungi. Results Nineteen strains of fungi with antimicrobial activity were attributed to four genera, five families, seven orders, and eight species. In these fungi, there were four strains with antimicrobial activity to E. coli（the max d/D value was 1.07）, Fifteen strains with antimicrobial activity to S. aureus（the max d/D value was 0.65）, and three strains with antimicrobial activity to C. albicans（the max d/D value was 1.27）. Conclusion Many kinds of fungi with antimicrobial activity inhabit in G. littoralis. This study is helpful to develop the recourses of endophytic fungi and to evaluate the genuineness of G. littoralis.

关 键 词：北沙参 内生真菌 抑菌活性 大肠杆菌 金黄色葡萄球菌 白色假丝酵母 真菌鉴定 

分 类 号：R282.23[医药卫生—中药学]