鼻咽癌细胞中EBER、GALC、CyclinD1表达的意义  

Significance of EBER, GALC and CyclinD1 expression in nasopharyngeal carcinoma cells

作　　者：王志静[1] 肖泽斌[1] 王付强[1] 郭莹[1] 李汝佳[1] 赵颖海[1] 陈小毅[1] 

机构地区：[1]广东医学院基础医学院病理学教研室,广东湛江524023

出　　处：《广东医学院学报》2015年第4期399-402,共4页Journal of Guangdong Medical College

基　　金：国家重点基础研究发展计划(973计划)资助(No.2011CB504800);广东省医学科学技术研究基金项目(No.A2015582)

摘　　要：目的探讨鼻咽癌细胞中EBER和GALC、Cyclin D1蛋白表达的意义。方法收集13例鼻咽黏膜慢性炎标本作为对照组,55例未分化型非角化性鼻咽癌标本作为实验组,用原位杂交法检测EBER表达,用免疫组化SP法检测GALC和Cyclin D1蛋白的表达。结果对照组和实验组中,EBER的表达阳性率分别为0%(0/13)和94.55%(52/55),GALC蛋白的表达阳性率分别为69.23%(9/13)和34.55%(19/55),Cyclin D1蛋白的表达阳性率分别为46.15%(6/13)和81.82%(45/55),组间差异均有统计学意义(P<0.01或0.05)。55例鼻咽癌中GALC与Cyclin D1蛋白的阳性表达呈负相关(r=-0.285,P<0.05)。结论 EBER、GALC、Cyclin D1可能在鼻咽癌的发生发展中起重要作用。Objective To investigate the significance of EBER, GALC and Cyclin D1 protein expression in nasopharyngeal carcinoma cells. Methods 15 cases of nasopharyngitis were collected as control group and 55 cases of undifferentiated nonkeratinized nasopharyngeal carcinoma（NPC） were collected as control and experimental groups, respectively. Expression of EBER was detected by in situ hybridization. Expression of GLAC and Cyclin D1 protein was detected by immunhistochemical SP method. Results In control and experiment groups, positive expression rate of EBER was 0%（0/13） and 94.55%（52/55）, respectively（P〈0.01）. Positive expression rate of GALC protein was 69.23%（9/13） and 34.55%（19/55） in two groups, respectively（P〈0.05）. Positive expression rate of Cyclin D1 protein was 46.15%（6/13） and 81.82%（45/55） in two groups, respectively（P〈0.05）. It showed a significant negative correlation between the expression of GALC and Cyclin D1 protein in 55 cases of NPC（r=-0.285, P〈0.05）. Conclusion EBER, GALC, Cyclin D1 may play important roles in the occurrence and development of NPC.

关 键 词：鼻咽肿瘤 EBV编码小RNAs 半乳糖(基)神经酰胺酶 细胞周期蛋白D1 

分 类 号：R739.63[医药卫生—肿瘤]