上调miR-34c抑制胶质瘤细胞的迁移及侵袭  

Effects of miRNA-34c on migration and invasion in human glioma cell line U251 and U87 in vitro

作　　者：武震东[1,2] 任洪波[3] 孙志强[3] 刘斌[3] 牛国栋[3] 焦保华[1] 

机构地区：[1]河北医科大学第二医院神经外科,石家庄市050000 [2]河北省邯郸市中心医院 [3]河北省邯郸市中心医院神经外科

出　　处：《河北医药》2015年第19期2888-2891,共4页Hebei Medical Journal

基　　金：河北省医学科学研究重点课题(编号:ZD20140280)

摘　　要：目的研究miR-34c对人脑胶质瘤细胞株U251和U87的迁移和侵袭的影响。方法应用脂质体将miR-34c导入胶质瘤细胞株U251和U87后,应用定量PCR验证转染效果,应用Transwell小室检测细胞迁移、侵袭能力变化。结果在U87和U251细胞系中,miR-34c-3p和miR-34c-5p的表达量要明显小于正常胶质细胞系HEB。而转染了miR-34c-3p的U87和U251细胞,其miR-34c-3p的表达量明显高于未转染组的细胞(Normal组,P<0.05)和NC组细胞(P<0.05),miR-34c-5p也呈同样的变化。利用Transwell小室,U251细胞转染miR-34c-3p或miR-34c-5p后,其迁移至下室的细胞数量与Normal组和NC组比较显著减少,在U87细胞中的情况相同。结论转染上调miR-34c在胶质瘤中的表达能够抑制其迁移和侵袭能力,为脑胶质瘤靶向治疗提供可靠的依据。Objective To investigate the effects of miRNA-34 c on migration and invasion in human glioma cell line U251 and U87 in vitro. Methods After miR-34 c was introduced into human glioma cell line U251 and U87 with liposome,the transfection efficiency was identified by quantitative PCR,and the changes of cell migration and invasion abilities were detected by Transwell chamber. Results In U87 and U251 cell lines,the expression levels of miRNA-34c-3p and miRNA-34c-5p were significantly lower than those in normal glial cells line HEB. However the expression levels of miRNA-34c-3p after transfection were significantly higher than those in normal glial cells and NC cells（ P〈0. 05）,so were miRNA-34c-5p.After miR-34c-3p or miR-34c-5p was transfected into U251 cells or U87 cells by means of Transwell chamber,the cell numbers which migrated into the lower chamber were significantly lower than those in normal cell group and NC group.Conclusion The up- regulation of expression of miRNA-34 c in glioma through transfection can inhibit the migration and invasion abilities of glioma,which provides a reliable basis for targeted therapy of brain glioma.

关 键 词：胶质瘤 miR-34c-3p miR-34c-5p 迁移 侵袭 

分 类 号：R739.4[医药卫生—肿瘤]