草酸脱羧酶促进草酸钙结晶溶解的体外研究  

Oxalate Decarboxylase Promotes the in vitro Dissolution of Calcium Oxalate Crystals

作　　者：贺俊斌[1] 龙寒[1] 林日辉[2] 梁宇薇 陈阳阳[1] 高华[1] 黄奇良[2] 

机构地区：[1]广西民族大学海洋与生物技术学院,广西高校微生物与植物资源利用重点实验室,广西南宁530006 [2]广西民族大学化学化工学院,化学与生物转化过程新技术广西高校重点实验室,广西南宁530006

出　　处：《食品科学》2015年第19期159-162,共4页Food Science

基　　金：教育部留学回国人员科研启动基金项目(教外司留[2012]1707号);广西自然科学基金项目(2014jjAA20030)

摘　　要：在体外实验条件下,考察了加酶量、反应pH值、反应温度及其产物甲酸根等因素对草酸脱羧酶催化草酸降解从而促进草酸钙结晶重新溶解过程的影响。结果表明,在10 mL反应体系中,添加4 U草酸脱羧酶,反应pH 3.0,反应温度为37℃,反应进行1周(168 h),可使近5%的草酸钙结晶重新溶解;实验结果表明,草酸降解产物甲酸根对草酸脱羧酶促进草酸钙结晶溶解产生抑制作用。Crystallization of calcium oxalate in the urinary system is one of the important causes of urinary stones,and enzymatic degradation of oxalic acid for preventing calcium oxalate stones has become a research hotspot.Under in vitro experimental conditions,the factors that affect the re-dissolution of calcium oxalate crystals catalyzed by oxalate decarboxylase were studied.The results indicated that when 4 U of oxalate decarboxylase was added to the 10-mL reaction system which was subsequently allowed to react at pH 3.0 and 37 ℃ for a week,the re-dissolution rate of calcium oxalate crystals was approximately 5%.The experimental results showed that the degradation product formate produced an inhibitory effect on the re-dissolution of calcium oxalate crystals catalyzed by oxalate decarboxylase.This experiment provides a theoretical reference for the study of enzyme preparation to control calcium oxalate stones.

关 键 词：草酸脱羧酶 草酸钙 溶解 

分 类 号：Q814[生物学—生物工程]