马尾松树皮提取物体外诱导肺腺癌GLC-82细胞凋亡的机制（英文）  

Mechanism of apoptosis of lung adenocarcinoma GLC-82 cell in vitro induced by Pinus massoniana bark extract

作　　者：张锦宏[1,2] 冯冬茹[1] 谢衡 李若达 王金发[1] 刘兵[1] 

机构地区：[1]中山大学生科院生物防治和基因工程教育部重点实验室,广州510275 [2]广州医科大学医学遗传与细胞生物学教研室,广州511436 [3]广州嵩珍营养源研究所,广州510645

出　　处：《中华中医药杂志》2015年第11期4134-4140,共7页China Journal of Traditional Chinese Medicine and Pharmacy

基　　金：Key Industry Project of Guangdong Province(No.2004b10401044);Natural Science Foundation of Guangdong Province(No.S2013040015594);Project for Doctoral Degree in Guangzhou Medical University(No.2012C15)~~

摘　　要：目的:探讨马尾松树皮提取物(Pinus massoniara bark extract,PMBE)在体外抑制肺腺癌GLC-82细胞生长的机制。方法:通过CCK-8法分析了PMBE对GLC-82细胞的生长活力影响;AO/EB染色、透射电镜和流式细胞术检测了PMBE诱导的肺腺癌细胞GLC-82的凋亡;Western blot检测PMBE处理GLC-82细胞后caspase-8、caspase-9、caspase-3、Bcl-2和Bid蛋白表达水平的变化。结果:PMBE在体外抑制肺腺癌GLC-82细胞的生长具有时间和剂量依赖性;通过荧光显微镜、透射电镜和流式细胞术证实PMBE诱导了GLC-82细胞的凋亡;细胞周期分析结果说明PMBE可以显著减少S期的GLC-82细胞;免疫杂交发现PMBE可以诱导caspase-3、caspase-8、caspase-9蛋白的激活,并下调Bcl-2和Bid蛋白的表达。结论:PMBE可能通过激活caspase-8从而激活Bid蛋白,进一步激活caspase-9来诱导肺腺癌GLC-82细胞的凋亡。Objectives： To research the inhibitory effects of Pinus massoniana bark extract（PMBE） on the lung adenocarcinoma GLC-82 cell in vitro. Methods： The CCK-8 method was used to analyze the inhibitory effect of PMBE on the growth of GLC-82 cells. The apoptosis of GLC-82 cell was measured by AO/EB staining, transmission electron microscopy, and flow cytometry. The expressions of caspase-8, caspase-9, caspase-3, Bcl-2 and Bid proteins were detected by Western blot method. Results： The growth of GLC-82 cells could be inhibited by PMBE that dependent on the concentration and time. PMBE could induce the apoptosis of GLC-82 cells that was confirmed via fluoroscope, TEM and flow cytometry. Cell cycle analysis revealed that the number of GLC-82 cells in the S phase was decreased by the treatment of PMBE. The result of Western blot showed that both the extrinsic and intrinsic pathways of the caspase cascade were activated, including caspase-8, caspase-9 and caspase-3. In addition, the expression of the Bcl-2 and Bid proteins were decreased. Conclusion： PMBE could induce the apoptosis of GLC-82 cells via activating caspase-8 to cleave the intact Bid that was an enzyme to activate caspase-9.

关 键 词：马尾松树皮提取物 GLC-82 凋亡 CASPASE BID BCL-2 

分 类 号：R285[医药卫生—中药学]