中药提取物中α-葡萄糖苷酶抑制活性因子的筛选  

Screening α-Glucosidase Inhibitors from Extracts of Chinese Medicine

作　　者：蒲秀瑛[1] 任菁[1] 刘璐[1] 马小龙[1] 李海兵[1] 李晓玥[1] 

机构地区：[1]兰州理工大学生命科学与工程学院甘肃省中藏药筛选评价及深加工重点实验室,甘肃兰州730050

出　　处：《中医药学报》2015年第5期68-70,共3页Acta Chinese Medicine and Pharmacology

基　　金：国家自然科学基金项目(81260070);兰州理工大学科研基金项目(10-0089)

摘　　要：目的:从中药提取物中筛选α-葡萄糖苷酶抑制活性因子。方法:采用α-葡萄糖苷酶活性测定方法 PNPG法,以阿卡波糖为阳性对照,对采取不同提取方法制备得到的多糖,生物碱,黄酮和皂苷类化合物进行α-葡萄糖苷酶抑制活性筛选。结果:党参多糖和桑白皮黄酮对α-葡萄糖苷酶抑制效果较好,接近阿卡波糖,党参皂苷次之,其余组分活性微弱或无活性。结论:本实验为今后进一步从党参和桑白皮中分离纯化出高活性的α-葡萄糖苷酶抑制活性因子提供了科学依据。Objective： To screen α-glucosidase inhibitors from natural products. Methods： In this experiment,PNPG was used to examine the activity of α-glucosidase,compared with the positive drug（ Acarbose） reaction rate,screening α-glucosidase inhibitors from polysaccharides,alkaloids,flavonoids and saponins. Results： The result indicated codonopsis pilosula polysaccharide and Cortex Mori flavonoid had a better inhibitory effect on α-glucosidase,close to the acarbose,and the second was codonopsis pilosula saponin,and other components were weak activity or inactive. Conclusion： The paper provides a scientific basis for the further separation and purification of α-glucosidase inhibitors from codonopsis pilosula and Cortex Mori.

关 键 词：党参 桑白皮 阿卡波糖 Α-葡萄糖苷酶 α-葡萄糖苷酶抑制因子 

分 类 号：R285[医药卫生—中药学]