高气压培养环境对胃癌SGC7901细胞PHD3表达的影响  

The effect of high-pressure culture environment on PHD3 expression in gastric cancer SGC7901 cells

作　　者：宿长磊[1] 刘文智[2] 郑宏群[3] 孙凌宇[3] 佟金学[3] 王天[2] 姜晓峰[4] 梁红艳[4] 薛丽[4] 张岂凡[3] 

机构地区：[1]哈尔滨医科大学附属第二医院普外科,150001 [2]哈尔滨工程大学信息与通信工程学院智能平台研究所,150001 [3]哈尔滨医科大学附属第四医院肿瘤外科,150001 [4]哈尔滨医科大学附属第四医院检验科,150001

出　　处：《国际遗传学杂志》2015年第5期242-248,共7页International Journal of Genetics

基　　金：黑龙江省自然科学基金（ZD200920）

摘　　要：目的本文从高气压培养环境对胃癌细胞PHD3基因表达的影响人手，进而探讨压力对肿瘤细胞分化的影响。方法本研究设计并研制了“调压式培养箱”，用于对培养的细胞施加持续性静压力或间歇性静压力。在实验中把1640培养液培养的胃癌SGC7901细胞分别在正常气压、加压760mmHg和加压1520mmHg的条件下，37℃培养30min。通过RT—PCR和Western印迹方法检测了胃癌SGC7901细胞中PHD3基因和蛋白表达的变化。结果实验结果证明，本研究设计并研制的调压式培养系统能够完成设计所需实验，通过调节空气和二氧化碳的比例，可以有效地维持细胞培养液的pH值不发生显著变化（P=0．6213）。通过将正常气压组、加压760mmHg组和加压1520mmHg组的实验结果经过统计学分析对比，发现高气压培养环境能够抑制PHD3蛋白的表达（P=0．0087）。结论本研究显示高气压培养环境能够抑制PHD3蛋白的表达，从而可能抑制SGC7901细胞的分化。Objective To evaluate effects of high pressure on PHD3 expression and differentiation of cancer cells. Methods To increase the extracellular pressure, we designed and developed a pressure adjustab.le incubator, which could maintain not only the pH of culture solution by regulating the ratio of air and carbon dioxide （CO2 ） but also the temperature through heating system. In this study, SGC7901 cells were incubated in RPMI 1640 and divided into three groups. After incubation for 30 minutes at 37℃ under ambient （760 mmHg） and increased pressure （1520 mmHg） conditions, ceils were harvested for detection of PHD3 and lS-actin expression by Western blot or RT-PCR. Results By RT-PCR and Western blot, we found that the expression of PHD3 decreased significantly. Conclusion In conclusion, high extracellular pressure suppresses expression of PHD3 and may inhibit SGC7901 cells differentiation.

关 键 词：胃癌 高气压培养 分化 PHD3 

分 类 号：R735.2[医药卫生—肿瘤]