X-射线照射对乳腺癌小鼠实验性肺转移的影响  

Effect of X-ray Irradiation on Experimental Lung Metastasis in Mice with Breast Cancer

作　　者：关江锋[1] 胡作为[1] 杨航[1] 李娜 

机构地区：[1]武汉市第一医院肿瘤科,武汉430022 [2]武汉市第五医院,武汉430050

出　　处：《华中科技大学学报（医学版）》2015年第5期552-555,共4页Acta Medicinae Universitatis Scientiae et Technologiae Huazhong

摘　　要：目的利用放射诱导乳腺癌小鼠肺损伤,探讨放射诱导的肺损伤对乳腺癌小鼠实验性肺转移的影响。方法采用MA782细胞株建立小鼠乳腺癌模型。28只建模后的C57BL/6小鼠随机分为2组：对照组和照射组（每组n=14）,右肺单次照射9Gy后1~4周,观察记录其体重变化及肺、肝、脾等脏器转移情况;苏木精-伊红（HE）染色观察肺组织形态学改变;免疫组化检测肺组织中趋化因子CXCL12/CXCR4的表达。结果照射后的1~2周,两组肝重、脾重和肺重变化无显著性差异（均P〉0.05）;然而,随着照射时间的推移,照射组小鼠在3~4周肺重、肺结节和肺脏指数明显增加（P〈0.05或P〈0.01）。肺组织形态学观察显示,在照射后的早期（1~2周）出现炎性细胞浸润;在照射后的第3~4周出现更多的肺转移性结节;肺泡壁增厚明显,肺间质各种细胞成分增加;支气管壁及血管外膜有明显的纤维化增厚,肺泡区域有纤维化形成。照射组肺组织中CXCL12/CXCR4表达显著高于对照组。结论 X-射线照射诱导的乳腺癌小鼠放射性肺损伤加速了肺转移,CXCL12-CXCR4生物信号轴在肿瘤发展、侵袭和肺转移中发挥重要作用。Objective To establish the radiation-induced lung injury model in mice with breast cancer,explore the effect of X-ray irradiation on lung metastasis in these mice and observe whether the radiation-induced lung injury will accelerate lung metastasis.Methods The breast cancer model was established with MA782 cells in C57BL/6 mice by intraperitoneal injection.Twenty-eight C57BL/6mice after modeling were randomly divided into 2groups：control group and irradiation group（n=14in each group）.In irradiation group,the right chest was irradiated with a dose of 9Gy one time when tumors grew to about 2mm×2mm in diameter.One to four weeks after the irradiation,the body weight was measured and liver,lung and spleen metastases were observed.HE staining was used to observe the morphological changes of lung tissues and the expression of chemokine factor CXCL12/CXCR4 in lung tissues was immunohistochemically detected.Results There was no statistical significance in the liver weight,spleen weight and lung weight between the two groups 1or 2weeks after the irradiation（P〈0.05）.With the irradiation time extended,especially in 3-4weeks,lung weight,lung nodules and lung index in the irradiation group were significantly increased（P〈0.05 or P〈0.01）.Lung tissue morphology showed that the inflammatory cell infiltration was found in the early time（1-2weeks）of irradiation;lung metastatic nodules were significantly increased 3-4weeks after irradiation;the alveolar wall was thickened and various cellular components in pulmonary interstitial tissues were obviously increased;fibrosis developed in the bronchial wall and vascular adventitia;fibrosis was found in the alveolar region.The expression level of CXCL12/CXCR4 was much higher in lung tissues in irradiation group than in control group.Conclusion X-ray irradiation-induced lung radioactive injury accelerates lung metastasis in mice with breast cancer.CXCL12-CXCR4 biological signal axis may play an important role in tumor development,invasion and metastasis.

关 键 词：照射 肿瘤移植 肺转移 CXCL12/CXCR4 

分 类 号：R737.9[医药卫生—肿瘤]