三氧化二砷对Burkitt淋巴瘤细胞株Raji凋亡的诱导作用及C-myc表达的影响  

Role of arsenic trioxide induced apoptosis in Burkitt lymphoma cell line Raji and influence on C-myc expression

作　　者：张继青[1] 钟雷[2] 李进娥[2] 

机构地区：[1]广州军区武汉总医院放射治疗科,湖北武汉430070 [2]广州军区武汉总医院门诊部,湖北武汉430070

出　　处：《临床荟萃》2015年第10期1123-1126,共4页Clinical Focus

摘　　要：目的探讨三氧化二砷(As2O3)对Burkitt淋巴瘤细胞株Raji凋亡的诱导作用及C-myc表达的影响。方法通过噻唑蓝比色法检测观察As2O3对Raji细胞增殖的影响,流式细胞仪观察As2O3对Raji细胞凋亡的诱导作用,逆转录聚合酶链式反应(RT-PCR)法检测As2O3对C-myc mRNA表达的影响。结果 As2O3对Raji细胞生长有抑制作用,呈剂量和时间依赖关系(P<0.01);As2O3对Raji细胞有诱导凋亡作用,呈剂量和时间依赖关系(P<0.01);随着As2O3浓度升高,C-myc的表达水平明显下降(P<0.01)。结论 As2O3对Burkitt淋巴瘤细胞株Raji具有增殖抑制和诱导凋亡作用,其作用机制可能与C-myc表达水平明显下降有关。Objective To investigate the role of arsenic trioxide（As2O3）induced apoptosis in Burkitt lymphoma cell line Raji and the influence on C-myc expression.Methods The influence of As2O3 on proliferation of Raji cells was detected by MTT colorimetric detection.The role of As2O3 induced apoptosis in Raji cells was detected by flow cytometry.The influence of As2O3 on the C-myc mRNA expression was detected by RT-PCR.Results As2O3 could inhibit the growth of Raji cells,and there was a dose-and time-dependent relationship（P 0.05）;As2O3 could induce the apoptosis of Raji cells,and there was a dose-and time-dependent relationship（P 0.05）;With the concentration of As2O3 increased,the expression of C-myc significantly decreased（P 0.05）.Conclusion As2O3 could inhibit the growth of Raji cells and induce the apoptosis of Raji cells.Its mechanism may be associated with lower C-myc expression.

关 键 词：三氧化二砷 淋巴瘤 凋亡 C-MYC 

分 类 号：R733.41[医药卫生—肿瘤]