柠檬酸钠强化剩余污泥酶水解和酸化的研究  

Enzymatic hydrolysis and acidification of excess sludge enhanced by sodium citrate

作　　者：罗琨[1] 杨麒[2] 李小明[2] 庞娅[1] 李雪[1] 廖兴盛[1] 裴习君[1] 

机构地区：[1]长沙学院生物与环境工程系,长沙410003 [2]湖南大学环境科学与工程学院,长沙410082

出　　处：《环境科学学报》2015年第11期3552-3557,共6页Acta Scientiae Circumstantiae

基　　金：国家自然科学基金(No.51308076);长沙市科技计划项目(No.k1407019-11);可再生能源电力技术湖南省重点实验室开放基金资助项目(No.2012ZNDL007)~~

摘　　要：厌氧条件下,研究了阳离子络合剂柠檬酸钠(SC)对剩余污泥酶水解和酸化的影响.结果表明:络合剂SC提高了污泥酶水解和酸化的效率,溶解性蛋白质和碳水化合物溶出量增加,SC的最佳投加剂量为0.432 g·g-1(以TS计,下同).络合剂SC可以提高污泥中短链脂肪酸(SCFAs)的积累量,同时减少达到最大SCFAs积累的时间,缩短厌氧消化时间.空白对照组和蛋白酶组的总SCFAs积累量分别在反应第7 d和第6 d达到最大值,而SC+蛋白酶组(SC 0.432 g·g-1)在反应第2 d就达到了最大值.从酶活性的变化和SEM图可知,SC的投加破坏了EPS的网络结构,原来被束缚、隐藏于污泥基体中的水解酶得到释放,从而提高了污泥水解速率.Effects of cation-binding agent sodium citrate（ SC） on excess sludge hydrolysis and acidification under anaerobic condition were investigated.Results showed that the efficiency of enzymatic hydrolysis and acidification of excess sludge could be greatly improved by cation-binding agent SC,and the release of soluble protein and carbohydrate was increased. The optimal SC dosage was 0.432 g·g-1TS. In addition,the accumulation of short-chain fatty acids（ SCFAs） increased significantly in the presence of SC,and a shorter fermentation time was required to reach the maximum SCFAs. The maximum SCFAs accumulation for blank and protease was achieved respectively on the seventh and sixth day,while it was on the second day for SC＋protease（ SC0.432 g·g-1）. According to the variation of enzyme activity and SEM observation,SC contributed to the disruption of flocs and the release of enzymes entrapped by or bound to the sludge structure,thus promoting the solubilization of sludge.

关 键 词：阳离子络合剂 柠檬酸钠 剩余污泥 酶水解 

分 类 号：X703[环境科学与工程—环境工程]