一株海洋生境芽孢杆菌FA08的筛选、鉴定及其酶学特性和抗菌性能分析  

SCREENING AND IDENTIFICATION, ENZYME CHARACTERISTICS, AND ANTIBACTERIAL ACTIVITY OF A MARINE BACILLUS STRAIN FA08

作　　者：方卫东[1,2] 唐旭[3] 刘源森[3] 林凌[3] 黄仕新 高培丽[3] 徐长安[3] 

机构地区：[1]集美大学水产学院,厦门361021 [2]福建省海新集团有限公司,漳州363102 [3]国家海洋局第三海洋研究所,厦门361005

出　　处：《海洋与湖沼》2015年第5期1228-1234,共7页Oceanologia Et Limnologia Sinica

基　　金：福建省科技计划项目;2013Y0063号;厦门南方海洋研究中心项目;13GZP002NF08号

摘　　要：利用生防细菌拮抗病原菌,是水产疾病防治的有效措施之一。本研究从福建省宁德市海水养殖区底泥筛选到一株既能产纤维素酶又具有拮抗水产病原菌功能的芽孢杆菌FA08,通过传统的形态学、生理生化鉴定手段,并结合16S r DNA序列分析,鉴定功能菌FA08为地衣芽孢杆菌(Bacillus licheniformis,Gen Bank登录号:KM892857)。进一步考察功能菌FA08菌株所产的纤维素酶对p H值、温度和紫外线的敏感性发现,功能菌FA08所产纤维素酶对酸碱度具有很强的耐受力,p H 3至p H 10范围内保持稳定,对温度也有较好的稳定性,在30—80°C范围内,其酶活仍达到70%,对紫外线不敏感。对FA08的抗菌性能进行分析,结果发现,功能菌FA08发酵上清对嗜水气单胞菌、哈维氏弧菌、溶藻弧菌、副溶血弧菌及金黄色葡萄球菌有拮抗效果。因此,该菌对未来水产业具有良好的开发应用价值。To develop biocontrol technology with bacterium against pathogens for prevention from aquatic diseases, we screened a bacillus strain FA08 from mud in an aquaculture area in Ningde, Fujian, China. The strain could produce with cellulase and showed antimicrobial activity, and has been identified as Bacillus licheniformis（Gen Bank Accession number KM892857）, based on its 16 S r DNA sequence homology, together with the morphological, physiological and biochemical characteristics. Analysis of its enzyme characteristic showed that the cellulase could stand in a wide p H range from 3 to 10 and remain thermally stable in 30—80°C with relative cellulase activity up to 70%. Moreover, the cellulase was insensitive to UV. In addition, we tested the antimicrobial activity of FA08 against selected aquatic pathogens. The results revealed that supernatant of the strain could inhibit strongly against Aeromonas hydrophila, Vibrio harveyi, Vibrio alginolyticus, Vibrio parahaemolyticus, and Staphylococcus aureus. Therefore, strain FA08 and its cellulase product shall have promising values of development and application for aquaculture.

关 键 词：地衣芽孢杆菌 纤维素酶 筛选鉴定 酶活特性 抗菌活性 

分 类 号：Q93-331[生物学—微生物学]