c-Met在结直肠癌细胞株中的表达及其配体肝细胞生长因子对SW480细胞株增殖和侵袭能力的影响  

Expression of c-Met in Colorectal Carcinoma Cells and Effect of Hepatocyte Growth Factor on Proliferation and Invasion of Colon Carcinoma Cells SW480

作　　者：姚建锋[1] 孙学军[2] 阎立昆[1] 贺赛[2] 李小军[1] 仝聪[1] 王小强[1] 

机构地区：[1]陕西省人民医院普通外科,陕西西安710068 [2]西安交通大学附属第一医院普通外科,陕西西安710061

出　　处：《中国普外基础与临床杂志》2015年第11期1314-1318,共5页Chinese Journal of Bases and Clinics In General Surgery

基　　金：国家自然科学基金项目(编号:81172362);陕西省自然科学基金研究计划项目(编号:2013JM4003)~~

摘　　要：目的探讨c-Met在结直肠癌细胞株中的表达以及其配体肝细胞生长因子(HGF)对结肠癌SW480细胞系增殖、侵袭能力的影响。方法应用RT-PCR及Western blot技术检测HGF受体c-Met在不同结直肠癌细胞中的表达,筛选出c-Met高表达细胞系;用不同浓度(0、20、40、70 ng/m L)的HGF作用SW480细胞48 h,分别用MTT法、Transwell法检测其对SW480细胞增殖、侵袭能力的影响。结果 1 RT-PCR及Western blot检测结果均表明,c-Met在不同结直肠癌细胞系中均有表达,其中体外培养的结肠癌细胞系SW480中存在c-Met和蛋白高表达。2 MTT实验结果显示,HGF可增强SW480细胞的增殖能力,且在一定范围内随浓度增高作用增强,呈剂量依赖性关系。3 Transwell实验结果显示,HGF处理后的SW480细胞侵袭能力增强。结论 c-Met在结肠癌SW480细胞中存在高表达,HGF可促进结直肠癌细胞的增殖及增加其体外侵袭能力。Objective To study the expression of c-Met in colorectal carcinoma cells and the effect of hepatocyte growth factor（HGF） on proliferation and invasion of colon carcinoma cells SW480.Methods Real-time PCR and Western blot methods were respectively used to detect the expressions of c-Met mRNA and protein in the different colorectal carcinoma cells in order to screen the high c-Met expression cells.The SW480 cells were incubated with different concentrations（0,20,40,and 70 ng/mL） HGF.MTT assay and Transwell test were used to evaluate the effects of proliferation and invasion in the SW480 cells.Results ① The c-Met was expressed in each colorectal carcinomar cells,especially highly expressed in the colon carcinoma cells SW480 in vitro.② MTT assay showed that the HGF could promote the proliferation of SW480 cells in a dose-dependent manner with some extent.③ Transwell test showed that the HGF could increase the invasion of SW480 cells.Conclusion The c-Met is highly expressed in colorectal carcinoma cells and HGF could promote proliferation and increase invasion of colorectal carcinoma cells in vitro.

关 键 词：C-MET蛋白 结直肠肿瘤 肝细胞生长因子 增殖 侵袭 

分 类 号：R735.34[医药卫生—肿瘤]