高渗盐水预处理对大鼠肝脏缺血再灌注损伤血清一氧化氮和内皮素-1表达水平的影响  

Effects of Hypertonic Saline on Levels of Nitric Oxide and Endothelin-1 in Hepatic Ischemia-Reperfusion Injury Rats

作　　者：黄涛[1] 尔启东 邓锋[1] 毕旭东[2] 

机构地区：[1]天津市西青医院普外科,天津300380 [2]辽宁医学院附属第一医院普外科,辽宁锦州121001

出　　处：《中国普外基础与临床杂志》2015年第11期1323-1326,共4页Chinese Journal of Bases and Clinics In General Surgery

摘　　要：目的探讨高渗盐水预处理对大鼠肝脏缺血再灌注损伤一氧化氮(NO)、内皮素-1(ET-1)表达水平的影响及其相关性分析。方法清洁健康雄性SD大鼠45只,随机分为假手术组、肝脏缺血再灌注组和高渗盐水预处理组3组,每组15只。用Pringle’s法建立大鼠肝脏缺血再灌注模型,检测肝脏缺血30 min再灌注1、6、24 h后血清NO、ET-1的水平,探讨缺血再灌注6 h时的NO、ET-1水平的相关性。结果肝脏缺血30 min再灌注1、6、24 h后,缺血再灌注组和高渗盐水预处理组血清NO水平均明显低于假手术组(P<0.01),而血清ET-1水平则均明显高于假手术组(P<0.01);高渗盐水预处理组血清NO水平明显高于缺血再灌注组(P<0.01),而血清ET-1水平则明显低于缺血再灌注组(P<0.01)。各组血清NO和ET-1水平在1、6、24 h之间的变化以6 h最为显著。大鼠血清NO与ET-1水平呈负相关(r=-0.970,P<0.01)。结论高渗盐水预处理改变了缺血再灌注损伤后血清NO与ET-1的水平,且二者水平的变化具有相关性,其作用机理可能是通过某种途径激发血清NO的水平、降低ET-1的水平,使两者的动态平衡向良性方向发展而达到保护作用。Objective To explore the effect of hypertonic saline（HTS） pretreatment on levels of nitric oxide（NO）and endothelin-1（ET-1） and their correlation in hepatic ischemia reperfusion（HIR） injury in rats.Methods The HIR injury models were made by using Pringle＇s maneuver in 45 healthy adult male Sprague-Dawley rats,which were randomly divided into three groups（n=15）：sham operation（SO） group,HIR group,and HTS group.The animals were killed at 1,6,and 24 h after reperfusion.The levels of serum NO and ET-1 were measured respectively,the correlation between NO level and ET-1 level at 6 h after reperfusion was analyzed.Results At the time points of 1 h,6 h,and 24 h after reperfusion,the serum NO levels in the HTS group and HIR group were all significantly lower than those in the SO group（P〈0.01）,but the serum ET-1 levels were all significantly higher than those in the SO group（P〈0.01）.The serum NO levels at the time points of 1 h,6 h,and 24 h in the HTS group were significantly higher than those at the same time in the HIR group（P〈0.01）,but the serum ET-1 levels in the HTS group were significantly lower than those in the HIR group（P〈0.01）.At all the time points,every detected goal had more marked level at the time point of 6 h after reperfusion.The NO level was negatively correlated with the ET-1 level.Conclusions HTS could change levels of serum NO and ET-1 after HIR injury,and which has a negative correlation.Its mechanism might probably stimulate serum NO level and reduce the ET-1 level through some way so as to enable both dynamic balance to the benign development direction and achieve a protective effect.

关 键 词：大鼠 肝脏 缺血再灌注损伤 高渗盐水 一氧化氮 内皮素-1 

分 类 号：R575[医药卫生—消化系统]