结直肠癌患者β-纤维蛋白原基因多态性和血浆纤维蛋白原水平相关性的研究  

The correlation study of beta-fibrinogen gene polymorphisms and plasma fibrinogen concentration in patients with colorectal cancer

作　　者：王静文[1] 韩韬[1] 陈玺[1] 唐曦[1] 

机构地区：[1]复旦大学附属华东医院肿瘤科,上海200040

出　　处：《中国癌症杂志》2015年第10期807-811,共5页China Oncology

基　　金：上海市卫生计生委员会科研资助项目(20114209)

摘　　要：背景与目的:结直肠癌患者病程中常伴有血浆纤维蛋白原(fibrinogen,Fg)浓度的升高。该研究探讨结直肠癌人群中FGBβ基因-448G/A、-148C/T、-1420G/A、-854G/A的单核苷酸多态性(single nucleotide polymorphism,SNP)频率分布和血浆Fg浓度的关系,分析其对肿瘤发生、发展的影响。方法:采集194例结直肠癌患者和74例健康者外周血,用实时荧光定量多聚酶链反应(real-time fluorescence quantitative PCR,RTFQ-PCR)法分析FGBβ基因频率分布,用Clauss凝固法检测血浆Fg浓度。结果:结直肠癌转移组和无转移组的血浆Fg浓度均较对照组显著升高(P<0.05)。疾病组中FGBβ-148T等位基因频率和突变型基因频率均显著高于对照组(P<0.05)。各组人群中FGBβ-148T携带者的血浆Fg浓度均明显高于非携带者(P<0.05)。在Ⅳ期患者中FGBβ-148C/T突变型者中位PFS较野生型者差异无统计学意义(P>0.05)。结论:结直肠癌患者体内Fg浓度升高,提示其可能参与恶性肿瘤发生、发展的过程。FGBβ-148C/T位点的SNP是导致血浆Fg浓度改变的原因之一,但与Ⅳ期患者的预后无关。Background and purpose：Patients with colorectal cancer are often accompanied by the increase of plasma ifbrinogen concentration. This study aimed to investigate the distribution characteristics of beta-ifbrinogen gene-448G/A, -148C/T, -1420G/A and -854G/A polymorphism and plasma ifbrinogen （Fg） concentration in patients with colorectal cancer. Furthermore, we analyzed their effects on the occurrence and development of cancer.Methods：The level of plasma Fg was quantiifed by using Clauss clotting method.FGBβ gene polymorphisms were identiifed by re-al-time lfuorescence quantitative PCR （RTFQ-PCR） in 194 colorectal cancer patients and 74 healthy controls.Results：The plasma Fg levels in tumor metastasis group and non-metastasis group were signiifcantly higher than that in control group, respectively （P〈0.05）. Compared with control group, the frequencies of -148T allele and mutation genotype were notably higher in disease group （P〈0.05）. In all the groups, the plasma Fg levels of those with -148T allele were higher than those without -148T allele （P〈0.05）. In stageⅣ patients, there was no difference in PFS between -148T wild gen-otype group and mutation genotype group （P〉0.05）.Conclusion：Plasma Fg concentration in patients with colorectal cancer was signiifcantly raised, which suggests that Fg may play a role in the occurrence and development of colorectal cancer. The beta-ifbrinogen gene -148C/T polymorphism is one of the reasons that cause plasma Fg elevation, but has no correlation with prognosis of patients with stageⅣ colorectal cancer.

关 键 词：纤维蛋白原 基因多态性 结直肠癌 

分 类 号：R735.35[医药卫生—肿瘤]