奥曲肽对人肝星状细胞凋亡及Bcl-2/Bax表达的影响  

Effects of octreotide on the apoptosis of human HSCs and expression of Bcl-2 / Bax in HSCs

作　　者：李春艳[1] 贾丽萍 石蕾[1] 周贤[1] 

机构地区：[1]泸州医学院附属医院消化内科,四川省646000 [2]第九人民医院内科,重庆市400700

出　　处：《实用医学杂志》2015年第20期3340-3342,共3页The Journal of Practical Medicine

基　　金：四川省卫生厅项目(编号:080181)

摘　　要：目的:观察奥曲肽对活化人肝星状细胞凋亡和凋亡相关蛋白Bcl-2/Bax表达的影响,探讨奥曲肽抗肝纤维化可能的作用机制。方法:不同浓度的奥曲肽作用于传代的人肝星状细胞株(HSC-LX2)24 h、48 h后,应用FITC-TUNEL检测各组细胞凋亡,应用免疫细胞化学法检测HSC-LX2中Bcl-2、Bax蛋白表达,应用Western-blot法检测HSC-LX2中Bcl-2蛋白表达。结果:奥曲肽可促进HSC-LX2细胞调亡,细胞凋亡率随奥曲肽浓度增加而增高(P<0.05);与24 h比较,相同浓度奥曲肽作用于HSC-LX2 48 h细胞凋亡率均明显增高(P<0.05);免疫细胞化学结果显示:与对照组相比,奥曲肽明显降低HSC-LX2中Bcl-2蛋白表达,而增加Bax蛋白表达(P<0.05);Western-blot结果表明:与对照组相比,奥曲肽可明显降低HSC-LX2中Bcl-2蛋白表达(P<0.05)。结论:奥曲肽可呈剂量和时间依赖性促进人肝星状细胞凋亡,其机制可能与奥曲肽诱发人肝星状细胞Bcl-2蛋白表达下调,Bax蛋白表达上调有关。Objective To investigate the effects of octreotide on the apoptosis of human hepatic stellate cells （HSCs） and expression of Bcl-2/Bax in HSCs,and to reveal the mechanism underlying octreotide against hepatic fibrosis. Methods HSCs lines （HSC-LX2） were incubated with different concentrations of octreotide for 24 and 48 hours. Cell apoptosis was evaluated by Fitc-tunel fluorescence staining. Bcl-2 and Bax protein exoression in HSC-LX2 was detected by immunocytochemistry. Meanwhile, Bcl-2 protein of HSC-LX2 were detected by Western blot assay. Results Octreotide could promote the apoptosis of HSC-LX2, and the apoptosis＆amp;nbsp;rate was significantly increased with the concentration of octreotide（P 〈 0.05）. The HSC-LX2 were incubated with the same concentration of octreotide for 24 and 48 hours, the cell apoptosis rate of 48-hour octreotide treatment was significantly higher than that of 24-hour octreotide treatment （P 〈 0.05）. The immunocytochemistry result indicated that octreotide could significantly decrease Bcl-2 expression and increase Bax expression in HSC-LX2 （P〈0.05）; Western blot assay showed that octreotide could also significantly inhibit Bcl-2 expression in HSC-LX2 （P〈0.05）. Conclusions Octreotide could induce the apoptosis of HSCs in a dose-and time-dependent manner, the mechanism of octreotide inducing HSCs apoptosis might be associated with down-regulation of Bcl-2 and upregulation of Bax in HSC.

关 键 词：奥曲肽 人肝星状细胞 凋亡 Bcl-2 

分 类 号：R575.2[医药卫生—消化系统]