基于HOOF-Prints技术的布鲁菌疫苗株基因序列遗传变异分析  

Analysis of genetic changes in Brucella vaccine strains by HOOF-Prints technique

作　　者：唐莉娟[1] 刘君[2] 陈创夫[3] 王远志[3] 张辉[3] 

机构地区：[1]巴音郭楞职业技术学院,新疆库尔勒841000 [2]四川理工学院生物工程学院,四川成都610000 [3]石河子大学,新疆石河子832003

出　　处：《中国预防兽医学报》2015年第11期891-893,共3页Chinese Journal of Preventive Veterinary Medicine

基　　金：国际科技合作项目(2006DFA33740);四川省教育厅科研项目(15ZB0210)

摘　　要：通过分析布鲁菌疫苗株基因序列遗传变异情况,为疫苗的免疫保护力的评价提供理论依据,本研究利用高变量八聚物寡核苷酸指纹技术(HOOF-Prints)对国内主要布鲁菌疫苗-羊种布鲁菌疫苗M5、猪种布鲁菌疫苗S2、牛种布鲁菌疫苗S19、人用布鲁菌疫苗104M进行序列分析,并基于布鲁菌标准参考株采用软件DNAMAN进行系统进化树分析。结果表明4种疫苗株的基因序列均发生了变异,但系统进化树分析显示这些疫苗株与对应的参考株遗传关系仍然最近,同时表明该指纹技术在分子水平上可以用于分析和了解国内主要疫苗的变异情况,为疫苗的免疫效果提供理论依据。To analyze genetic changes in Brucella vaccine strains, the B.melitensis M5, B.suis $2, B.abortus S19 and B.abortus 104M vaccine strains were analyzed based on hypervariable octameric oligonucleotide finger prints （HOOF-Prints）. Phylogenetic tree of the vaccine strains were aligned with their Brucella reference strains with the software DNAMAN, respectively. The results showed that the sequences of all four vaccines displayed divergence, although they were clustered into corresponding species. These findings implied that HOOF-Prints technique could be employed into analyzing the variation of major domestic vaccines and provide a theoretical basis for the immune effect of the vaccines.

关 键 词：HOOF—Prints 布鲁菌疫苗株 遗传进化 

分 类 号：S852.61[农业科学—基础兽医学]