基于小麦酯酶的有机磷农药残留快速检测研究  

Study of the Rapid Detection of Organophosphorus Pesticide Residues Based on Wheat Esterase

作　　者：魏进[1] 张盈[1] 段婷婷[1] 

机构地区：[1]贵州省农业科学院植物保护研究所,贵阳550006

出　　处：《价值工程》2015年第33期208-209,共2页Value Engineering

基　　金：基于植物源酯酶筛选农药残留快速检测试纸的研制与应用;项目编号:黔科合NY字【2012】3050号

摘　　要：研究了小麦酯酶检测有机磷农药的残留分析方法,根据酶抑制法的原理,对反应温度、pH、反应时间、显色剂及样品提取液等条件进行优化,得出采用固兰B盐既兼顾了底物和显色剂双重作用,又具有底物稳定性好、灵敏、简便实用的特点,同时也体现出固兰B盐法的优越性,其最低检出浓度为0.1mg/kg。This article studies the analysis method of using wheat esterase to detect organophosphorus pesticide residues. According to the principle of enzymatic inhibition method, the reaction temperature, p H, reaction time, and the agent sample extracts are optimized. It is concluded that using fast blue salt B can not only considers the dual function of the substrate and the chromogenic agent, but also has the features of stable, sensitive and practical substrate, and at the same time the advantages of fast blue salt B are shown. Its minimum detectable concentration is 0.1mg/kg.

关 键 词：小麦酯酶 检出 有机磷农药 

分 类 号：TS210.7[轻工技术与工程—粮食、油脂及植物蛋白工程]