油田采出水微生物处理菌株选育及特性研究  

Breeding and Characteristics Research of the Microbial Strains for Oilfield Produced Water Treatment

作　　者：杜茂林[1] 谷亚楠[1] 付瑞敏[2] 陈五岭[1] 

机构地区：[1]西北大学生命科学学院 [2]河南教育学院人口与生命科学系

出　　处：《油气田环境保护》2015年第5期26-30,91,共5页Environmental Protection of Oil & Gas Fields

基　　金：陕西省重大科技创新项目;编号2009ZKC04-16

摘　　要：为解决油田采出水微生物处理菌株的应用局限,通过He-Ne激光和紫外线复合诱变,选育出能够在油田采出水中快速生长,并有效降解油田采出水主要危害成分的5种优势菌株。经形态学和生理生化鉴定,这5株菌分别为枯草芽孢杆菌(Bacillus subtilis)、醋酸钙不动杆菌(Acinetobacter calcoaceticus)、多食鞘氨醇杆菌(Sphingobacterium multivorum)、荧光假单胞菌(Pseudomonas fluorescens)和解脂假丝酵母(Candida tropicalis)。经检验,所筛选的菌株均能降解石油烃类以及聚丙烯酰胺类物质,并对高碱、高温环境有一定耐受性,可将其直接应用于油田采出水的处理。In order to solve the pollution problem of oilfield produced water,five strains,which can grow rapidly and degrade main harmful substances efficiently,were selected by using He-Ne laser and UV compound mutation.After the colony morphology and physiological and biochemical test,the five strains were identified as Bacillus subtilis,Acinetobacter calcoaceticus,Sphingobacterium multivorum,Pseudomonas fluorescens and Candida tropiealis.After testing,the strains,which were able to survive under high temperature and alkali environment and degrade petroleum hydrocarbons and polyacrylamide substance,can be applied directly to the treatment of oilfield produced water.

关 键 词：油田采出水 微生物处理 菌株选育 降解特性 

分 类 号：X741[环境科学与工程—环境工程]