血清miR-210和CA199联合检测在胰腺癌诊断中的应用  

Application of combined detection of serum miR-210 and CA199 in the diagnosis of pancreatic carcinoma

作　　者：陈文举[1,2] 陈美芳[3] 王攀[2] 周勇[2] 沈波[3] 

机构地区：[1]温州医科大学,浙江温州325000 [2]台州市中心医院检验科,浙江台州318000 [3]台州医院检验科,浙江台州317000

出　　处：《中国卫生检验杂志》2015年第20期3481-3483,共3页Chinese Journal of Health Laboratory Technology

基　　金：台州市科技计划项目(1201ky10);台州恩泽医疗(中心)集团恩泽科研基金(12EZD15)

摘　　要：目的研究胰腺癌患者血清miR-210的表达及意义,探讨血清miR-210与CA199联合检测在胰腺癌早期诊断中的价值。方法选取37例胰腺癌患者和40例健康对照者,应用实时荧光定量PCR检测血清中miR-210的表达水平;化学发光法检测血清中CA199含量;受试者曲线(ROC)分析miR-210、CA199及两者联合对胰腺癌的诊断价值。结果与对照组相比,胰腺癌患者血清中miR-210表达上调,CA199含量增加,差异均有统计学意义(P<0.05)。ROC分析结果显示,miR-210诊断胰腺癌的敏感度和特异度分别为80.9%和91.3%,均高于CA199(敏感度、特异度分别为63.2%、88.9%),miR-210和CA199联合检测的敏感度(95.5%)较单项指标明显提高。结论胰腺癌患者血清miR-210的表达上调,miR-210诊断胰腺癌的敏感度和特异度均高于CA199;两指标联合可明显提高诊断的敏感度。血清miR-210有潜力成为胰腺癌早期诊断的分子标志物之一。Objective To investigate the value of combined detection of serum miR-210 and CA199 in the early diagnosis of pancreatic carcinoma. Methods 37 patients with pancreatic cancer patients and 40 healthy controls were included in our study. Real-time fluorescence quantitative PCR was used to detect the expression level of miR-210 in serum and the expression level of serum CA199 was detected by chemiluminescence method. The ROC analysis of miR-210,CA199 and their combination in the diagnosis of pancreatic cancer were performed. Results Compared with normal controls,the expression of miR-210 in serum of patients with pancreatic cancer was upregulated,CA199 content increased,the difference was statistically significant（ P〈0. 05）. The ROC analysis results showed that the sensitivity and specificity of miR-210 in the diagnosis of pancreatic cancer were 80. 9% and 91. 3%,respectively,which were higher than that of CA199（ sensitivity and specificity was63. 2%,88. 9%,respectively）. The sensitivity（ 95. 5%） of the combination detection of miR-210 and CA199 was significantly improved compared with the single index. Conclusion The serum levels of miR-210 were up-regulated in pancreatic cancer patients. The sensitivity and specificity of miR-210 in diagnosis of pancreatic cancer were higher than those of CA199.Combination of the two index can improve the sensitivity of diagnosis. Serum miR-210 has the potential to be one of molecular markers for the early diagnosis of pancreatic cancer.

关 键 词：胰腺癌 微小RNA-210 实时荧光定量PCR CA199 

分 类 号：R735.9[医药卫生—肿瘤]