基因敲除技术在炎症性肠病肠上皮屏障机制研究中的应用  

Application of gene knockout technology in research of intestinal epithelial barrier mechanism in infl ammatory bowel disease

作　　者：陈柳[1] 吴焕淦[2] 施茵[2] 

机构地区：[1]上海中医药大学岳阳临床医学院,上海市201203 [2]上海市针灸经络研究所,上海市200030

出　　处：《世界华人消化杂志》2015年第29期4673-4679,共7页World Chinese Journal of Digestology

基　　金：国家自然科学基金资助项目;Nos.81273844;81473757;国家重点基础研究发展计划(973计划)基金资助项目;No.2015CB554500~~

摘　　要：肠上皮屏障通透性改变/增加所致的肠上皮屏障损伤在炎症性肠病发病机制中起重要作用,正常肠上皮屏障通透性的维持依赖于跨上皮细胞途径和细胞间紧密连接途径这两方面.近年来,随着分子生物学技术的发展以及各种基因工程技术的广泛应用,通过基因敲除技术特异性敲除某一特定基因来研究疾病的发病机制成为热点.本文综合了近年来国内外相关研究文献,旨对基因敲除技术在炎症性肠病肠上皮屏障的跨上皮途径和紧密连接途径这两方面研究中的应用进行简要概述,试图对深入开展炎症性肠病的发病机制研究提供一些思路.Intestinal epithelial barrier permeability changes/increase caused by intestinal epithelial barrier damage play an important role in the pathogenesis of inflammatory bowel disease,and the maintenance of normal intestinal epithelial barrier permeability depends on the two aspects of the trans-epithelial cell pathway and the tight connection between the cells.In recent years,with the development of molecular biology technology and wide application of a variety of gene engineering technology,specific knocking out a particular gene through gene knockout technology to study the pathogenesis of the disease has become a hot research topic.In this paper,we review the application of gene knockout technology in the research of intestinal epithelial barrier trans-epithelial pathway and tight junction pathway in inflammatory bowel disease,in order to provide some ideas for further study of the pathogenesis of inflammatory bowel disease.

关 键 词：基因敲除术 炎症性肠病 肠上皮屏障 综述 

分 类 号：R574[医药卫生—消化系统]