低氮胁迫下丛枝菌根真菌对垂穗披碱草的抗氧化系统调控机制  

Inoxidizability Regulatory Mechanism of Arbuscular Mycorrhizal Fungi on Elymus nutans under Low Nitrogen Stress

作　　者：李海英 孙永芳[2] 许岳飞[2] 杨云贵[2] 

机构地区：[1]陕西省畜牧技术推广总站,陕西西安710016 [2]西北农林科技大学动物科技学院草业科学系,陕西杨凌712100

出　　处：《家畜生态学报》2015年第10期61-65,共5页Journal of Domestic Animal Ecology

基　　金：国家牧草产业技术体系(CARS-35-01A);国家自然科学基金项目(31402129)

摘　　要：试验首先通过在石英砂中添加不同氮源来筛选出氮高效植株,再往垂穗披碱草根部接种丛枝菌根真菌来进一步提高其吸收有机氮的抗氧化性。研究结果表明,在三种有机氮中,野生型申扎垂穗披碱草(SZ)吸收谷氨酸的能力最强。当有机氮作为氮源时,两种垂穗披碱草的生长均受到了一定的影响,且北京正道垂穗披碱草(ZD)受到的影响较大。与未接种菌种相比,接种激活的丛枝菌根真菌能降低两种垂穗披碱草的电解质渗出率和丙二醛(MDA)含量,显著降低了两种垂穗披碱草的超氧化物歧化酶(SOD)、过氧化氢酶(CAT)和过氧化物酶(POD)活性,BJ分别降低了54.08%、15.82%和49.34%(P<0.05);SZ分别降低了75.11%、21.80%和40.03%(P<0.05)。总之,接种AM真菌有助于提高垂穗披碱草吸收有机氮的抗氧化性。To improve the inoxidizability of nitrogen in plants, Elymus nutans were identified through adding different nitrogen source in silica sand and applying arbuseular mycorrhizal fingi（AMF）. The resuits indicated that SZ Elymus nutans were the strongest in capturing glutamic acid among three organic nitrogen. When organic nitrogen was the source of nitrogen, two Elyrnus nutans were stressed to some extent, and BJ was weaker in growth. Application of AMF could decrease electrolyte leakage rate and malondialdehyde （MDA） content. It also decreased the activity of superoxide dismutase （SOD）, catalase （CAT） and Peroxidase （POD）in Elymus nutans. BJ decreased by 54.08 %, 15.82 %and 49.34 %, respectively（P 〈0.05）,andSZ decreased by 75.11% .21.80%and 40.03%, respectively（P〈0.05）. In conclusion, Application of active AMF could improve inoxidizability of organic nitrogen in Elymus nutans.

关 键 词：垂穗披碱草 丛枝菌根真菌 有机氮 抗氧化性 

分 类 号：S811.6[农业科学—畜牧学]