生长期膳食缺锌小鼠肾细胞凋亡及机制研究  

Study on the mechanism of apoptosis of mouse kidney cells in dietary zinc deficiency during the growth period

作　　者：田娟[1] 郭芳[1] 李晓明[1] 

机构地区：[1]辽宁医学院组织学与胚胎学教研室,121001

出　　处：《天津医药》2015年第11期1281-1283,共3页Tianjin Medical Journal

基　　金：辽宁省科学技术计划项目(2013022067);辽宁省教育厅科学技术研究项目(L2012307);辽宁省大学生创新创业训练计划项目(201410160037);国家级大学生创新创业训练计划项目(201410160037);辽宁省高等学校优秀人才项目(LJQ2013092)

摘　　要：目的观察膳食缺锌条件下生长期小鼠肾细胞凋亡情况、氧化应激情况及凋亡相关因子Bcl-2和Bax的表达变化,探讨锌缺乏诱发肾细胞凋亡的机制。方法将刚断乳雄性小白鼠30只随机分为缺锌组和足锌组,每组15只。缺锌组喂饲缺锌饲料(0.85 mg/kg),足锌组喂饲足锌饲料(30 mg/kg)。应用TUNEL法观察小鼠肾细胞凋亡情况,计算凋亡指数;分别应用黄嘌呤氧化酶法和硫代巴比妥酸法测定肾超氧化物歧化酶(SOD)和丙二醛(MDA)含量;应用蛋白印迹技术检测肾组织中Bcl-2、Bax蛋白表达变化。结果与足锌组比较,缺锌组小鼠肾可见TUNEL阳性细胞增多,细胞凋亡指数明显增加;抗氧化酶SOD活性下降,MDA增加,氧化应激反应增强;凋亡相关因子Bcl-2蛋白表达量明显下降,Bax蛋白表达量明显上调,Bcl-2/Bax比值下降。结论生长期膳食缺锌导致肾细胞抗氧化酶活性降低,氧化应激反应增强及细胞凋亡相关因子Bcl-2和Bax蛋白表达改变,最终导致肾细胞发生凋亡。Objective To observe the cell apoptosis, oxidative stress reaction and expressions of Bcl-2 and Bax in kid-ney of dietary zinc deficiency mice during growth period, and discuss the mechanism of renal cell apoptosis induced by zinc deficiency. Methods Thirty weaning male mice were randomly divided into zinc-deficient group and zinc-adequate group, and 15 mice for each group. Zinc-deficient group was fed with zinc deficiency diet （0.85 mg/kg）, while zinc-adequate group was fed with enough zinc diet （30 mg/kg）. The TUNEL method was applied to observe the cell apoptosis, and the apoptotic in-dex was measured. The content of SOD and MDA were detected to observe the oxidative stress reaction in kidney. The ex-pression levels of Bcl-2 and Bax protein were detected by Western blot assay. Results Compared with zinc-adequate group, the cell apoptosis and oxidative stress reaction were increased in zinc-deficient group. The expression of Bcl-2 de-creased, and the expression of Bax increased. The ratio of Bcl-2 and Bax declined in kidney of zinc deficiency mice. Conclu-sion Diet zinc deficiency in growth period may result in the decreased antioxidase, the increased oxidative stress reaction, and the changed Bcl-2 and Bax expression, which promote the occurrence of cell apoptosis in kidney.

关 键 词：锌 细胞凋亡 肾 氧化性应激 BCL-2相关X蛋白质 缺锌 

分 类 号：R737.1[医药卫生—肿瘤]