口腔鳞状细胞癌中FEZ1和HIF-1α蛋白表达及预后研究  

Expressions of FEZ1 and HIF-1α protein in oral squamous cell carcinoma and its prognosis

作　　者：张晋弘[1] 刘俊英[2] 刘健[3] 刘月平[2] 

机构地区：[1]河北医科大学第一医院口腔科,050031 [2] 河北医科大学第四医院病理科 [3] 河北医科大学第四医院口腔科

出　　处：《天津医药》2015年第11期1311-1314,I0005,共5页Tianjin Medical Journal

基　　金：河北省卫生厅科研基金项目(项目编号20100281)

摘　　要：目的探讨抑癌基因FEZ1和缺氧诱导因子(HIF)-1α在口腔鳞状细胞癌(OSCC)中的表达及其与临床病理特征及预后的关系。方法选取OSCC组织标本146例,正常黏膜组织标本80例,同时选取新鲜OSCC组织、癌旁组织和正常黏膜组织各30例。免疫组化法检测146例组织标本和80例正常黏膜组织中FEZ1和HIF-1α表达,分析二者表达与患者临床病理特征及预后的关系。Western blot检测30例新鲜OSCC组织及其对应癌旁组织、正常黏膜组织中FEZ1和HIF-1α表达情况。结果 146例OSCC组织FEZ1蛋白阳性表达率(30.14%)低于正常口腔黏膜组织(81.25%),HIF-1α蛋白阳性表达率(71.23%)高于正常黏膜组织(12.50%,χ2分别为54.076和71.317,均P<0.01)。146例OSCC中FEZ1的表达水平与临床分期、肿瘤直径、分化程度有关,HIF-1α的表达水平与性别、淋巴结转移、肿瘤直径、分化程度有关。FEZ1α蛋白阳性表达与阴性表达患者5年生存率差异无统计学意义,而HIF-1α蛋白阳性表达者5年生存率低于阴性表达患者(P<0.05)。30例正常口腔黏膜组织、癌旁组织及OSCC组织中,FEZ1表达水平依次降低(P<0.01),HIF-1α表达水平依次升高(P<0.01)。结论 FEZ1和HIF-1α可能参与了OSCC的发生、发展,检测FEZ1和HIF-1α表达可作为OSCC生物学行为的参考指标之一。Objective To investigate the expressions of tumor suppressor gene FEZ1 and hypoxia inducible factor （HIF）-1α in oral squamous cell carcinoma （OSCC）, and their correlation with clinicopathological features and prognosis. Methods A total of 146 specimens of OSCC and 80 normal oral mucosa were used to detect the expressions of FEZ1 and HIF-1αby immunohistochemistry. Thirty specimens of fresh OSCC tissues, 30 samples of the paracancerous tissues and 30 normal mucosa tissues were also included in the study. The relationship between the expressions of FEZ1 and HIF-1α, the clinicopathological features and the prognosis of the patients were analyzed. Western blot assay was used to detect the expres？sions of FEZ1 and HIF-1αin 30 fresh OSCC tissues, 30 samples of the paracancerous tissues and 30 normal mucosa tissues. Results In OSCC tissues, the positive rate of FEZ1 protein （30.14%） was significantly lower than that in normal oral muco？sa （81.25%）, and the positive rate of HIF-1αprotein （71.23%） was significantly higher than that in normal mucosa （12.50%,χ2=54.076 and 71.317, P〈0.01）. There was a correlation between the expression of FEZ1 and clinical stage, tumor size and differentiation. The expression of HIF-1αwas correlated with gender, lymph node metastasis, tumor size and differentiation. There was no significant difference in 5-year survival rate between positive and negative FEZ1 expression groups. The 5-year survival rate was significantly lower in HIF-1α positive expression group than that of HIF-1α negative expression group （P〈0.05）. The expression level of FEZ1 was decreased by the sequences in normal oral mucosa, paracancerous tissue and OSCC （P〈0.01）, but the expression level of HIF-1αwas increased in turn （P〈0.01）. Conclusion FEZ1 and HIF-1αmay be involved in the occurrence and progress of OSCC. The expressions of FEZ1 and HIF-1αcan be used as a reference for the biological behavior of OSCC.

关 键 词：口腔鳞状细胞癌 FEZ1 缺氧诱导因子-1Α 免疫组织化学 蛋白免疫印迹 临床特征 生存分析 

分 类 号：R782[医药卫生—口腔医学]