Snail介导GOLPH3对人脑胶质瘤细胞迁移和侵袭作用的研究  

GOLPH3 promotes glioblastoma cell migration and invasion via Snail

作　　者：赵伟[1] 张旭 陆冬[2] 张昊[1] 石琼[3] 周秀萍[3] 于如同[2,3] 

机构地区：[1]徐州医学院研究生学院,徐州221002 [2]徐州医学院附属医院脑科医院神经外科 [3]徐州医学院神经系统疾病研究所

出　　处：《临床神经外科杂志》2015年第5期341-345,共5页Journal of Clinical Neurosurgery

基　　金：国家自然科学基金项目(81272777;81372699;81472345)

摘　　要：目的研究GOLPH3影响脑胶质瘤细胞的迁移和侵袭能力及机制。方法观察GFP荧光和Western blot鉴定过表达或下调GOLPH3的U251细胞;分别应用RT-PCR、Western blot实验检测GOLPH3对Y-box binding protein 1(YB1)蛋白及核酸水平、对MMP-2及Snail的调节;采用Snail siRNA下调Snail的表达后,Western blot检测Snail及YB1的水平,细胞划痕实验和Transwell侵袭实验检测Snail对细胞的侵袭迁移能力的影响;Western blot检测在过表达GOLPH3的U251细胞中下调Snail后,GOLPH3、Snail及YB1的水平;同时应用细胞划痕实验和Transwell侵袭实验检测此共转实验对细胞迁移和侵袭能力的影响。结果下调GOLPH3抑制YB1的蛋白与核酸水平以及MMP2核酸水平。下调GOLPH3抑制Snail的蛋白表达,过表达GOLPH3,Snail表达量增加。下调Snail后,YB1的表达量降低,并抑U251细胞的侵袭与迁移能力。过表达GOLPH3引起的细胞的迁移和侵袭能力增加被下调Snail所逆转。结论 GOLPH3通过Snail调节YB1的表达,调节U251细胞的迁移和侵袭能力。Objective To study the role and mechanism of Golgi phosphoprotein 3（ GOLPH3） in glioma cell migration and invasion. Methods The effect of GOLPH3 on the expression of YB1,snail and MMP2 was examined by Western blotting and RT-PCR respectively. After down-regulation of snail,the protein level of YB1 were analyzed by western blotting,cell migration and invasion ability were examined by wound healing and invasion assay respectively. After GOLPH3 over-expression with or without Snail down-regulation,GOLPH3,Snail and YB1 protein expression levels in U251 glioma cells were determined by western blotting,and the ability of cell migration and invasion were examined by wound healing and invasion assay respectively. Results Down-regulation of GOLPH3 decreased the protein and mRNA levels of YB1 and mRNA levels of MMP2. Down-regulation of GOLPH3 decreased the protein level of Snailwhile over-expression of GOLPH3 showed contrary effect.Down-regulation of Snail decreased the level of YB1 and inhibited the migration and invasion of U251 cells. Over-expression of GOLPH3 inducing U251 cell migration and invasion were blocked by Snail down-regulation. Conclusion Down-regulation of GOLPH3 inhibited glioma U251 cell migration and invasion through regulating Snail and YB1 expression.

关 键 词：脑胶质瘤 迁移 侵袭 GOLPH3 SNAIL YB1 

分 类 号：R739.41[医药卫生—肿瘤]