新吉细毛羊角蛋白的EST筛选及表达研究  

Screening and Expression of Keratin EST in Xinji Fine Wool Sheep

作　　者：刘春洁[1] 付雪峰[2] 田可川[2] 黄锡霞[1] 徐新明[2] 詹振宏[2] 于丽娟[2] 

机构地区：[1]新疆农业大学动物科学学院,乌鲁木齐830052 [2]新疆畜牧科学院畜牧研究所,新疆绒毛用羊遗传育种与繁殖实验室,乌鲁木齐830000

出　　处：《中国畜牧兽医》2015年第11期2873-2879,共7页China Animal Husbandry & Veterinary Medicine

基　　金：国家自然科学基金项目“超细型和细型细毛羊皮肤组织中基因的差异表达及其遗传效应研究”(31360543);十二五自治区重大专项“新疆毛、绒羊新品种选育与产业化示范”(201230116-6);国家农业科技成果转化资金项目“优质超细毛羊生产配套技术推广与示范”(2012GB2G400493);现代农业产业技术体系建设专项“绒毛用羊产业技术体系”(CARS-40)

摘　　要：试验旨在从新吉细毛羊皮肤组织表达序列标签(expressed sequence tags,ESTs)中筛选与毛囊发育相关基因并探讨其表达模式与羊毛细度的相关性。利用组装、比对软件Trinity与Blat对新吉细毛羊皮肤组织ESTs组装、注释进而筛选与毛囊发育相关基因,并通过实时荧光定量PCR相对定量方法以2-△△Ct值检测相关基因在不同绵羊个体中的表达规律。结果显示,从474条ESTs中筛选注释获得了4个与毛囊发育相关的角蛋白基因KRT27、KAP3.2、KAP11.1和KAP8.1;4个角蛋白基因在超细型细毛羊皮肤组织表达量极显著高于细型细毛羊的表达量(P<0.01);4个角蛋白基因表达量:KRT27>KAP3.2>KAP11.1>KAP8.1。该研究为角蛋白基因在细毛羊转录水平上的研究提供了一定数据。The experience was conducted to screen the genes associated with hair follicle development from the expressed sequence tags（ESTs）derived from the skin tissues of Xinji fine wool sheep and explore the association between their expression patterns and the fineness of wool.Using Trinity and Blat,we screened out the candidate genes by assembly and homologous annotation of ESTs,and further studied their expression patterns through detecting the correlation value2^-△△Ct by Real-time PCR method.As a result,four genes（KRT27,KAP3.2,KAP11.1and KAP8.1）were carried out to be responsible for hair follicle development,and 4keratin genes were extremely significant more highly expressed in skin tissues of super fine wool sheep than in fine wool sheep（P〈0.01）;The expression level of 4keratin were as follow：KRT27〉KAP3.2〉KAP11.1〉KAP8.1.The study provided certain data for the research on transcript level of fine wool sheep keratin gene.

关 键 词：EST序列 角蛋白基因 RT-PCR 显著表达 

分 类 号：S826[农业科学—畜牧学]