电穿孔与脂质体lipofectamine转染miR-218进入肝癌细胞效率的比较  

Comparison study of the transfection efficiency between electroporation and lipofectaminetransfection on human hepatocarcinoma cells

作　　者：蔡磊[1] 李艳 郭曼[1] 连肖[1] 杨学文[1] 王文斌[1] 

机构地区：[1]第四军医大学西京消化病医院,陕西西安710032 [2]西北妇女儿童医院麻醉科,陕西西安710061

出　　处：《现代肿瘤医学》2015年第24期3542-3544,共3页Journal of Modern Oncology

基　　金：国家自然科学基金资助项目(编号:81300301)

摘　　要：目的:比较采用电穿孔和脂质体lipofectamine两种方法转染人肝癌细胞的效率优劣。方法:分别采用电穿孔和脂质体转染miR-218进入人肝癌细胞Hep G2,采用RT-PCR、Western blot、流式细胞仪、Tunel检测等方法比较两种方法的转染后对人肝癌细胞的影响。结果:采用两种方法都可以成功将miR-218转染进人肝癌细胞Hep G2中,降低了Hep G2中miR-218靶基因RNA编辑酶ADAR1的表达,使得细胞凋亡增加,但电穿孔转染组效率更高。结论:电穿孔转染是一种安全、高效的转染方法,值得推广。Objective： To compare the transfection efficiencies of miR -218 into human hepatocarcinoma cells HepG2 by electroporation or lipofectamine. Methods： miR- 218 was transfected either by electroporation or by lipo- fectamine. Cells were collected, and then the condition of HepG2 cells were detected by RT - PCR, Western blot, Flow Cytometry and Tune1 test. Results：Both two methods could transfect miR -218 into HepG2 swimmingly,leading to the increase of apoptosis ,with the apporach of electroporation had abetter effect. Conclusion：Electroporation transfection is a safe. effective approach, which is worthy oromotion.

关 键 词：电穿孔 脂质体lipofectamine 转染 人肝癌细胞HEPG2 ADAR1 MIR-218 

分 类 号：R730[医药卫生—肿瘤] R735.7[医药卫生—临床医学]