原花青素对脑缺血再灌注损伤后肠道功能的保护作用  

Protective Effects of Proanthocyanidins on Intestinal Function after Cerebral Ischemia-reperfusion Injury

作　　者：范加维 杨森[1] 杨拯[1] 黄宏达[1] 陈勇[1] 宋强[1] 罗兰[1] 梁杰[1] 张婷[1] 黄倩[1] 

机构地区：[1]成都医学院,四川成都市610500

出　　处：《中国康复理论与实践》2015年第10期1138-1144,共7页Chinese Journal of Rehabilitation Theory and Practice

基　　金：国家大学生创新创业训练计划项目(No.201513705013);成都医学院大学生开放实验项目(No.Kf03)

摘　　要：目的探讨原花青素预处理对脑缺血再灌注损伤后肠道功能的保护作用。方法将24只Sprague-Dawley大鼠随机分为假手术组(A组,n=8)、缺血再灌注组(B组,n=8)和原花青素预处理组(C组,n=8)。参照Longa线栓法制备大鼠脑缺血再灌注损伤模型。C组腹腔注射原花青素10 mg/(kg?d),A组和B组注射等量生理盐水,连续5 d。分别于术后1 d、3 d,测定大鼠回肠肌电慢波和平滑肌收缩、血清超氧化物歧化酶(SOD)活性和血清丙二醛(MDA)含量;ELISA试剂盒测定血清TNF-α含量;取回肠组织进行苏木精-伊红(HE)染色和含水量测定。结果脑缺血再灌注1 d、3 d后,C组肠黏膜损伤较B组减轻,肠黏膜损伤评分降低(P<0.05),黏膜炎性细胞浸润数目减少;C组慢波和收缩频率有升高趋势(P>0.05),振幅较B组均增加(P<0.05);C组血清SOD活力增加(P<0.05),MDA含量和TNF-α含量明显下降(P<0.01),肠含水量明显降低(P<0.01)。结论原花青素预处理对脑缺血损伤后的肠道功能有保护作用。Objective To explore the protective effects of proanthocyanidins pretreatment on intestinal function after cerebral ischemia- reperfusion injury. Methods 24 Sprague- Dawley rats were randomly divided into sham group（group A, n=8）, ischemia- reperfusion group（group B, n=8） and proanthocyanidins pretreatment group（group C, n=8）. The model of cerebral ischemia-reperfusion injury in rats was established according to Longa＇s method. Group C was intraperitoneally injected with proanthocyanidins 10 mg/（kg · d）, group A and group B were injected with normal saline for 5 consecutive days. 1 and 3 days after cerebral ischemia-reperfusion, ileum myoelectric slow wave and smooth muscle contractility, the activity of superoxide dismutase（SOD） and content of malondialdehyde（MDA） were measured,the content of the serum TNF-α was tested with ELISA kit, ileum tissues were tested with hematoxylin eosin（HE） staining and used for measuring the moisture content. Results Compared with group B 1 and 3 days after cerebral ischemia-reperfusion, the intestinal mucosa injury relieved, the intestinal mucosa score decreased（P〈0.05） and the number of infiltrated inflammatory cell decreased in group C; the frequency of slow wave and contraction trended to increase（P〉0.05）, and the amplitude increased（P〈0.05） in group C; the serum SOD activity increased（P〈0.05）, and the content of MDA and TNF-α decreased（P〈0.01） in group C; the intestinal moisture content reduced（P〈0.01） in group C. Conclusion Proanthocyanidins pretreatment can protect intestinal function from injury after cerebral ischemia-reperfusion.

关 键 词：脑缺血再灌注 原花青素 肠道功能 大鼠 

分 类 号：R743.3[医药卫生—神经病学与精神病学]