Biglycan 及 FAK 信号通路对结肠癌细胞侵袭、转移能力的影响及机制  

Effects and mechanism of Biglycan and FAK signaling pathway on the invasion and metastasis of colon cancer cells

作　　者：谷小虎[1] 肖景东[2] 邢晓静[1] 

机构地区：[1]辽宁省肿瘤医院,沈阳110042 [2]辽宁中医药大学

出　　处：《实用肿瘤学杂志》2015年第5期444-449,共6页Practical Oncology Journal

基　　金：辽宁省自然科学基金（201102111）;国家自然科学基金项目（81201968）

摘　　要：目的 观察Biglycan对局部黏着斑激酶（ Focal adhesion kinase,FAK）活化的影响,探索其是否通过调控FAK信号通路影响结肠癌细胞的侵袭和迁移. 方法 构建过表达Biglycan的人结肠癌细胞系HCT116并施加FAK抑制剂处理. 实验设置空白对照组（HCT116）、空载体对照组（Vector）,空载体抑制剂处理组（Vector＋PF-562271）、Biglycan过表达组（Biglycan）和Biglycan过表达抑制剂处理组（Bigly-can＋PF-562271）. 抑制剂处理24 h后,采用Western blot技术检测FAK、p-FAK在各组结肠癌细胞中的表达;采用Transwell实验分析各组结肠癌细胞的侵袭和迁移能力. 结果 Biglycan过表达后会显著提高FAK的磷酸化水平,促进HCT116细胞的侵袭和迁移（P〈0.01）;FAK抑制剂PF-562271能够明显降低p-FAK的表达,逆转Biglycan对结肠癌细胞侵袭和迁移能力有影响（ P〈0.01）. 结论 Biglycan通过激活FAK信号通路影响结肠癌细胞系HCT116的侵袭和转移.Objective To observe the influence of Biglycan on the focal adhesion kinase（ FAK） activa-tion and to explore whether it regulates the invasion and metastasis of colon cancer through FAK signaling path-way.Methods The overexpressive plasmid of Biglycan was constructed and then transfected into the colon canc-er cell line HCT116.Meanwhile,FAK inhibitor was used to treat cells.Control group（HCT116）,empty plasmid group（Vector）,empty plasmid and inhibitor treatment group（Vector ＋PF -562271）,Biglycan overexpression group（ Biglycan） ,Biglycan overexpression and inhibitor treatment group（ Biglycan＋PF-562271） were set.Twen-ty four hours after exposure to inhibitor,the expression of FAK and p-FAK in each group was detected by West-ern Blot.The invasion and metastasis of colon cancer cells was detected by transwell assay.Results Overexpres-sion of Biglycan significantly enhanced the phosphorylation level of FAK and promoted the invasion and metastasis of HCT116（P〈0.01）.The inhibitor of FAK PF-562271 could significantly reduce the expression of p-FAK and reverse the effect of Biglycan on the invasion and metastasis of colon cancer cells.Conclusion Biglycan reg-ulates the invasion and metastasis of colon cancer cells through activation FAK signaling pathway.

关 键 词：BIGLYCAN 局部黏着斑激酶 结肠癌 侵袭 迁移 

分 类 号：R735.3[医药卫生—肿瘤]