大豆皮过氧化物酶提取条件的优化  

Optimization of Extracting Conditions of Peroxidase from Soybean Seed Coat

作　　者：邓祥宜[1] 李继伟[1] 孙欣欣[1] 伍校军 张兴国[1] 倪章如 

机构地区：[1]华中农业大学楚天学院食品与生物科技学院,武汉430205

出　　处：《食品工业》2015年第11期15-17,共3页The Food Industry

摘　　要：以黄豆为原料,对大豆皮过氧化物酶(SBP)的提取条件进行优化。通过单因素试验和正交试验考察水浴温度、液料比、水浴时间和提取次数对SBP得率的影响。结果显示SBP的最佳提取条件为液料比20︰1(m L/g),40℃水浴提取时间5 h,提取1次。此条件下的SBP得率为7.17×103 U/g。Using soybean as material,the optimization of the extraction conditions of soybean seed coat peroxidase（SBP）was done.Through single-factor tests and orthogonal test,the effects of several factors on SBP extraction rate were investigated,such as water bath temperature,liquid-powder ratio,water bath processing time and extraction cycles.The results showed that the optimal parameters of SBP extraction were liquid-powder ratio of 20：1（mL/g）,water bath processing 5 h under 40℃ and one extraction cycle.The SBP yield under the optimal conditions reached 7.17×10^3 U/g.

关 键 词：大豆皮过氧化物酶 提取条件 优化 

分 类 号：TS229[轻工技术与工程—粮食、油脂及植物蛋白工程]