HPLC测双氯芬酸钾干混悬剂的含量  

Determination of Diclofenac Potassium Dry Suspension Agent by HPLC

作　　者：隋丽丽[1] 孟祥军[1] 

机构地区：[1]沈阳医学院,辽宁沈阳110034

出　　处：《广州化工》2015年第21期116-118,共3页GuangZhou Chemical Industry

基　　金：辽宁省教育厅科学研究一般项目(L2012374)

摘　　要：建立了高效液相色谱法测定双氯芬酸钾干混悬剂含量的测定方法。色谱柱为Kromasil C18（250 mm×4.6 mm,2.5μm）,流动相：甲醇-水-冰醋酸（80∶20∶0.8,V∶V∶V）,检测波长为276 nm,流速为1.0 m L·min-1,进样量为20μL。结果表明：y=40999x＋119667（r=0.9995,n=7）线性范围为4.0~6.0μg·m L-1;最低检测限为1.66 ng,定量限为5.56 ng;平均回收率为99.57%。该方法简便、准确、适用于双氯芬酸钾干混悬剂的含量测定。The high performance liquid chromatography（ HPLC） method for the content of diclofenac potassium dry suspension agent was established to study the high performance liquid chromatography（ HPLC） method for the content of diclofenac potassium dry suspension agent,using chromatographic column for Kromasil C18（ 250 mm × 4. 6 mm,2. 5 μm）,mobile phase methanol-water-acetic acid glacial（ 80∶20∶0. 8）,detection wavelength of 276 nm,flow rate of 1. 0 m L·min-1,sample amount to 20 μL. Results showed that 40999x＋y = 40999（ r = 0. 9995,n = 7） with linear range of 4. 0 ~ 6. 0 μg·m L-1,the minimum detection limit was 1. 66 ng,quantitative limit was 5. 56 ng. The average recovery was 99. 57%. The method is suitable for determination of the diclofenac potassium dry suspension agent,and it is convenient,accurate,simple and feasible.

关 键 词：双氯芬酸钾 干混悬剂 含量测定 高效液相色谱法 

分 类 号：O657.72[理学—分析化学]