美金刚对异氟烷所致神经干细胞增殖能力减退的保护作用  

Protective Effects of Memantine on Isofluane-induced Decrease of Proliferation in Neural Stem Cells

作　　者：陈欣[1] 赵以林[1] 王伟[1] 张建芳[1] 李世勇[1] 罗爱林[1] 

机构地区：[1]华中科技大学同济医学院附属同济医院麻醉科,武汉430030

出　　处：《医药导报》2015年第11期1433-1438,共6页Herald of Medicine

基　　金：国家自然科学基金资助项目(81271233)

摘　　要：目的通过体外实验观察美金刚对异氟烷所致神经干细胞增殖能力减退的保护作用及其可能机制。方法从出生1 d的大鼠海马获取神经干细胞,体外培养并随机分为5组:对照组(control组)、溶剂对照组(Vehicle组)、异氟烷组(Iso组)、异氟烷+美金刚组(Iso+Mem组)和异氟烷+美金刚+LY294002组(Iso+Mem+LY组)。通过检测Brd U在增殖神经干细胞中的表达了解神经干细胞增殖水平,Western blot法检测神经元磷酸化Akt蛋白表达水平。结果 2.4%异氟烷暴露6 h后神经干细胞增殖能力和磷酸化Akt蛋白表达水平均显著下降(P<0.01)。美金刚可同时缓解异氟烷所致神经干细胞增殖能力减退和磷酸化Akt蛋白表达水平下降(P<0.01)。Akt通路特异性抑制剂LY294002可逆转美金刚对神经干细胞增殖的保护作用(P<0.01)。结论美金刚可通过激活Akt信号转导通路,缓解异氟烷所致神经干细胞增殖能力减退。Objective To investigate the protective effects of memantine on isofluane-induced decrease of proliferation in neural stem cells （ NSCs） and the potential mechanisms in vitro. Methods Neural stem cells were isolated from rat hippocampi （postnatal day 1） and grew in culture. Cultured NSCs were randomly divided into the control （ Group Control）, Vehicle （Group Vehicle）, Isoflurane （ Group Iso）, Isoflurane ＋Memantine （ Group Iso ＋Mem） and Isoflurane ＋ Memantine ＋LY294002 groups （Group Iso＋Mem＋LY）.Proliferation was assessed by cell counting and BrdU incorporation.Western blot was conducted to detect protein expression of phospho-Akt. Results Compared with the control group,BrdU incorporation and phospho-Akt expressions in neural stem cells significantly declined after 2.4% isoflurane exposure for 6 h （P〈0.01）.However, isofluane-induced decrease of BrdU incorporation and phospho-Akt expressions was attenuated by the treatments of memantine （P〈0.01））. It was showed that Akt inhibitors LY294002 reversed the protective effects on neural stem cells proliferation by memantine（P〈0.01）. Conclusion The results suggest that memantine treatment might attenuate isofluane-induced decrease of proliferation in neural stem cells via Akt signaling pathway.

关 键 词：美金刚 异氟烷 干细胞 神经 增殖 

分 类 号：R742.8[医药卫生—神经病学与精神病学] R965[医药卫生—临床医学]