金线莲正丁醇提取物降血糖作用  

Hypoglycemic Effect of n-butyl Alcohol Extraction from Anoectochilus roxburghii

作　　者：陈剑飞[1] 张晓春[1] 

机构地区：[1]湖北医药学院附属随州医院湖北省随州市中心医院药学部,随州441300

出　　处：《医药导报》2015年第11期1454-1457,共4页Herald of Medicine

摘　　要：目的研究金线莲正丁醇提取物的降血糖作用及其机制。方法以健康SD大鼠12只为正常对照组,将糖尿病模型大鼠48只随机分为模型对照组和金线莲正丁醇提取物大、中、小剂量组,每组12只。金线莲正丁醇提取物大、中、小剂量组分别灌胃给予金线莲正丁醇提取物600,400,200 mg·kg-1,正常对照组和模型对照组给予等体积0.9%氯化钠溶液,均每天1次。给药3周后,测定大鼠体质量、血清葡萄糖含量和血清胰岛素水平。结果实验结束时,与正常对照组比较,模型对照组大鼠体质量明显减轻(P<0.01),血清葡萄糖明显升高(P<0.01),与模型对照组比较,金线莲正丁醇提取物大、中、小剂量组体质量差异无统计学意义(P>0.05),血清葡萄糖明显降低,差异有统计学意义(P<0.01或P<0.05),金线莲正丁醇提取物大剂量组血清胰岛素水平明显升高(P<0.05)。结论金线莲正丁醇提取物具有显著降血糖作用,大剂量金线莲正丁醇提取物能显著增加大鼠胰岛素水平,其降糖机制可能与促进β细胞再生有关。Objective To study the hypoglycemic effect and mechanism of n-butyl alcohol extraction from Anoectochilus roxburghii. Methods The total of 12 normal rats were used as normal control group, and 48 diabetic model rats were divided into model control group, and high dose, middle dose and low dose treatment groups with 12 rats in each group,three treatment groups received Anoectochilus roxburghii at dose 600,400,200 mg·kg^-1 , respectively, normal control group and model control group were administered with equal volume of 0.9% sodium chlorade solution once a day.After 21 days of drug administration, the weights of rats, the levels of blood glucose and insulin were examined. Results Compared with normal control group, the body weight in model control group decreased significantly（P〈0.01）, while blood glucose significantly increased （P〈0.01）.Compared with model control group, the body weight in Anoectochilus roxburghii groups exhibited no obvious difference,while blood glucose decreased significantly （P〈0.01 or P〈0.05）.The level of insulin in high dose group increased remarkably （P〈0.05）. Conclusion The n-butyl alcohol extraction from Anoectochilus roxburghii shows markedly hypoglycemic effect,specifically for which at high dose increases the level of insulin significantly,its mechanism may involve with promoting β cells regeneration.

关 键 词：金线莲 正丁醇提取物 糖尿病 降血糖作用 

分 类 号：R286[医药卫生—中药学] R965[医药卫生—中医学]