超声波-酶法提取大杯蕈菇柄多糖及流变性研究  

Study on Ultrasonic-enzyme-assisted Extraction and Rheological Characteristics of Polysaccharides From Clitocybe maxima Stipes

作　　者：赖谱富[1] 方汝涛 陈君琛[1] 杨艺龙[1] 

机构地区：[1]福建省农业科学院农业工程技术研究所/福建省农业科学院农产品加工研究中心/国家食用菌加工技术研发分中心,福建福州350003

出　　处：《核农学报》2015年第10期1944-1953,共10页Journal of Nuclear Agricultural Sciences

基　　金：公益性行业(农业)科研专项(201303080);国家科技支撑计划项目(2012BAD14B15);福建省科技重大专项项目(2012NZ0002);福建省农业科学院青年基金项目(2012DG-3)

摘　　要：为了探讨大杯蕈菇柄多糖的流变学特性,利用响应面分析法对大杯蕈菇柄多糖的超声波-复合酶解提取工艺进行优化,建立了超声功率、超声时间、酶解温度、酶解p H和酶解时间的五因素回归模型,分析了模型的有效性与因子间的交互作用,并研究了浓度、温度、盐离子浓度、p H值、热处理、剪切作用等对大杯蕈菇柄多糖黏度的影响。结果表明:大杯蕈菇柄多糖提取最佳工艺条件为超声功率450W、超声时间15min、酶解温度45℃、酶解p H值4.5、酶解时间60min,在此条件下大杯蕈菇柄多糖得率可达5.59%;大杯蕈菇柄多糖溶液的黏度随其浓度的增加而增大,随其温度的升高而降低,酸性或碱性条件下均不同程度降低,对剪切作用、热处理均具有较好的稳定性。本研究可为大杯蕈菇柄多糖的提取及产品开发提供理论依据。The technique in extracting polysaccharides from Clitocybe maxima stipes was optimized with ultrasonic assisted enzymatic method to develop this technology for industrial promotion. Response surface method（ RSM） was used in estimation for setting up the regression model by five extraction factors,including ultrasonic power,ultrasonic time,the temperature,pHand time of zymolysis,as well as the interaction between those factors and the reliability of the regression model. The rheological character of stipe polysaccharides of Clitocybe maxima was discussed in its viscosity resulting of polysaccharide solution in concentration,temperature,sodium chloride concentration,pH,heating and shearing treatment. Results showed that optimal parameters in extractive factors were ultrasonic power 450 w,ultrasonic time 15 min,zymolysis temperature 45℃,zymolysis pH 4. 5,zymolysis time 60 min,respectively. With these conditions,the yield of polysaccharide extraction for Clitocybe maxima could reach to 5. 59%. The viscosity of polysaccharide solution increased with the increasing in polysaccharide concentration but the decreasing in solution temperature,it decreased somewhat at any acidic or alkaline conditions and performed good properties in resisting-heat and resisting-shearing action.

关 键 词：大杯蕈菇柄 多糖 提取 超声波 复合酶解法 流变性 

分 类 号：TQ281[化学工程—有机化工]