灰色关联度法筛选适合液体深层发酵灵芝菌株  

Grey Relational Analysis of Suitable Ganoderma Strains for Liquid Fermentation

作　　者：汤坤鹏 刘盛荣 吴小平[1] 仇志恒[1] 张亚楠[1] 

机构地区：[1]福建农林大学菌物研究中心,福建福州350002 [2]福建宁德师院,福建宁德352100

出　　处：《核农学报》2015年第10期1963-1969,共7页Journal of Nuclear Agricultural Sciences

基　　金：福建省种业创新与产业化工程专项(2014S1477-21)

摘　　要：为筛选灵芝液体深层发酵专用型菌株,以菌丝生物量、三萜、胞内及胞外多糖为评价指标,采用灰色关联度法综合评价。结果表明,24株灵芝菌株中HS-2生物量最高,达16.44 g·L-1;三萜含量以菌株J-16/AL-14最高(0.33%);胞内多糖以菌株H(韩芝)含量最高(10.13%),胞外多糖以菌株006(16.96 g·L-1)和S-1(16.11g·L-1)最高;此外,灵芝菌丝检测到灵芝酸C和F。以权重分别为生物量0.4、三萜0.2、胞内多糖0.2以及胞外多糖0.2进行灰色关联分析,显示菌株006与参考菌株关联度最高,适合作为灵芝液体深层发酵菌株。本研究将灰色关联度法应用于灵芝液体深层发酵菌株筛选,对灵芝工业化液体发酵生产具有参考价值。This study utilized Grey relational analysis to screen Ganoderma strains in liquid culture for their contents of biomass,triterpenoids,and intracellular and extracellular polysaccharides. The results showed that,strain HS- 2 had the highest biomass（ 16. 44 g·L^-1）,Strain J- 16 /AL- 14 yielded highest intracellular triterpenoid content,（ 0. 33%）,a maximum intracellular polysaccharide（ IPS） was found in strain H（ 10. 13%）,and strains 006 and S-1 produced most（ 16. 96 g·L^-1） extracellular polysaccharide（ EPS）. In addition,Ganoderic acids C and F were detected in the cells of all strains. With a Grey related biomass,triterpenoid,IPS and EPS of 0. 4,0. 2,0. 2 and 0. 2respectively,analysis showed that strain 006 was highest related to the reference strain,indicating that it was a desirable Ganoderma lucidum strain for industrial liquid culture. This study reports the beneficial application of Grey relational analysis for screening of Ganoderma strains for their suitability in industrial liquid fermentation.

关 键 词：灵芝 液体深层发酵 生物量 三萜 多糖 灰色关联度 灵芝酸 

分 类 号：S567.31[农业科学—中草药栽培]