^60Co-γ射线辐照柑橘大实蝇低级别蛹对成虫羽化和交配的影响  

Effect of Irradiating at Earlier Pupal Stages by ^(60)Co-γ Radiation on the Eclosion and Mating of Bactrocera minax( Enderlein) Adults

作　　者：黄聪[1,2] 王福莲[1,2] 马跃坤[1,2] 许弘毅 张桂芬[3] 李传仁[1,2] 

机构地区：[1]长江大学农学院 [2]长江大学昆虫研究所,湖北荆州434025 [3]中国农业科学院植物保护研究所/植物病虫害生物学国家重点实验室,北京100193

出　　处：《核农学报》2015年第11期2117-2122,共6页Journal of Nuclear Agricultural Sciences

基　　金：公益性行业(农业)科研专项经费项目(200903047);湖北省自然基金项目(2011CDB003);大学生创新创业训练计划项目(2014031)

摘　　要：为了降低柑橘大实蝇4级蛹在辐照时因成虫羽化造成的虫量损失,保证辐照虫的质量,方便辐照操作和缓解运输压力,以柑橘大实蝇低级蛹(1级、2级和3级)为试验材料,研究经90Gy的60Co-γ射线辐照后的成虫羽化率和交配能力。结果表明,辐照2级和3级蛹未对成虫羽化和交配产生显著影响,2级和3级蛹辐照后成虫羽化率分别为58.4%和60.4%;单雌(雄)交配次数分别为0.15次和0.08次;成虫羽化历期均为12 d,羽化高峰分别为5月19~20日和5月16~17日,与未辐照蛹和辐照的4级蛹的差异不显著;雌雄性比分别为0.91和1.04,与未辐照蛹无显著差异。1级蛹辐照后成虫雌雄性比为0.62,显著低于未辐照蛹和辐照的4级蛹,且羽化历期与未辐照蛹和辐照的4级蛹相比缩短了6 d。综合衡量辐照效应和经济效益,可以将柑橘大实蝇的适宜辐照虫期扩展至2、3级蛹。本研究为高效应用柑橘大实蝇辐照不育技术奠定了理论基础。In order to reduce the adult losses caused by irradiation on the forth stage pupae of Bactrocera minax and be convenient to handle and assure the quality of B. minax adults,the eclosion rate and mating capacity of adults irradiated at the earlier pupal stages（ 1st,2nd and 3rd pupal stages） were studied. The results showed that there was no significant effect on the eclosion and mating capacity of adults irradiated at the 2nd and 3rd pupal stages,the eclosion rate of adults was 58. 4% and 60. 4%,mating numbers of single female and male were 0. 15 and 0. 08,respectively,the eclosion period was 12 d,eclosion peak of adults was May 19 to 20 and May 16 to 17,respectively. There were no significant differences between the non-irradiated and irradiated pupae at 4th pupal stage. Sex ratio was 0. 91 and 1. 04,respectively,no significant differences when compared with non-irradiated pupae. While the sex ratio of adults irradiated at the first pupal stage was significant lower（ 0. 62） than that of non-irradiated or irradiated 4th stage pupae,and the eclosion period was shortened by 6 d compared to the pupae non-irradiated or irradiated at 4th pupae stage. Combine the irradiation effect and economic profit,the suitable irradiated stage of B. minax also could be the 2nd and 3rd pupal stages. The results could lay the theoretical foundation for the efficient application of B. minax irradiation sterile technique.

关 键 词：^60Co-γ射线 柑橘大实蝇 辐照效应 辐照虫期 

分 类 号：S436.66[农业科学—农业昆虫与害虫防治]