利用405nm激光激发Hoechst33342染色细胞DNA的流式细胞技术  

405 nm Laser Exciting Hoechst33342 in Detection of Cell DNA Content by Flow Cytometry

作　　者：户乃丽[1] 徐晓雪[1] 邹林樾[1] 杨巍巍[2] 

机构地区：[1]首都医科大学医学实验与测试中心,北京100069 [2]首都医科大学宣武医院老年病研究所神经生物学研究室,北京100069

出　　处：《现代生物医学进展》2015年第33期6406-6409,共4页Progress in Modern Biomedicine

基　　金：国家自然科学基金青年基金项目(81401042)

摘　　要：目的:探讨流式细胞仪上405 nm激光激发Hoechst33342染色细胞DNA的效果及影响检测结果的因素。方法:SW480和A549两种细胞经Hoechst33342染色后,流式细胞仪405 nm激光激发检测DNA含量,利用软件计算出处于G0/G1期、S期和G2/M期细胞的百分比,以PI染色法结果作为对照。结果:SW480和A549细胞经Hoechst33342染色后各期的细胞百分比与PI染色法基本一致,无明显差异(P>0.05)。结论:405 nm激光激发Hoechst33342染色细胞DNA结果可靠,可作为紫外检测的替代方法。Objective： To observe the effect and influential factors of flow cytometry with 405 nm laser using Hoechst33342 in detection of DNA content. Methods： SW480 and A549 cells were stained with Hoechst33342 and then excited by 405 nm laser to detect DNA content, percentages of cells in G0/G1, S and G2/M phases were calculated by software,results of PI staining method were used for control. Results： The percentages of SW480 and A549 cells in each period stained with Hoechst33342 were basically same with PI staining method（P 〉0.05 ）. Conclusion： 405 nm laser is credible in exciting Hochest33342 staining cell DNA, so it can replace ultraviolet laser to detect DNA content.

关 键 词：405 nm激光 HOECHST33342 流式细胞术 细胞周期 

分 类 号：R-331[医药卫生]