醒脑静注射液联合血府逐瘀汤对SAH大鼠CRP、TNF-α的影响  

Effect of Xing Nao Jing Injection Combined with XueFuZhuYu Decoction on the Changes of CRP and TNF-α in Rats with Subarachnoid Hemorrhage

作　　者：朱文锐[1] 张云会[1] 田俊[1] 刘建仁[1] 胡鹏[1] 戴先才[1] 

机构地区：[1]广州中医药大学第一附属医院颅脑科,广东广州510405

出　　处：《现代生物医学进展》2015年第33期6410-6414,共5页Progress in Modern Biomedicine

基　　金：2013年广东省中医药局课题(20132126)

摘　　要：目的:探讨醒脑静注射液联合血府逐瘀汤对蛛网膜下腔出血(subarachnoidhemorrhage,SAH)大鼠C反应蛋白(C-reactionprotein,CRP)、肿瘤坏死因子-a(Tumor necrosis factor-a,TNF-a)的影响。方法:50只大鼠随机分为5组:正常组,模型组,醒脑静组(0.3 m L/kg),血府逐瘀汤组(0.3 m L/kg),联合用药组(醒脑静联合血府逐瘀汤)。除正常组外,其余4组采用"二次枕大池注血法"建立大鼠SAH模型。从造模成功后的第1 d开始给药,连续给药7 d。正常对照组及模型组给予等量生理盐水。分别在术前、术后1 d、3 d、7 d、14 d取血浆及脑脊液,按ELISA试剂盒说明书分别测定各组血清及脑脊液中肿瘤坏死因子-α(TNF-α)、C-反应蛋白(CRP)的含量。结果:(1)术后随着时间推移,SAH大鼠血清及脑脊液中TNF-α及CRP含量逐渐上升,在第7 d达到高峰。(2)术后第1 d,与正常组比较,模型组中血清及脑脊液中TNF-α、CRP含量均显著上升,差异显著(P<0.01);与模型组比较,醒脑静组及血府逐瘀汤组中血清及脑脊液中TNF-α、CRP含量无差异(P>0.05),而联合用药组中血清及脑脊液中TNF-α含量下降,差异具有显著意义(P<0.05)。(3)术后第3 d、7 d、14 d,与正常组比较,模型组中血清及脑脊液中TNF-α、CRP含量均显著上升,差异显著(P<0.01);与模型组比较,醒脑静组、血府逐瘀汤组及联合用药组中血清及脑脊液中TNF-α、CRP含量下降,差异均显著(P<0.01),与醒脑静组或血府逐瘀汤组比较,联合用药组中血清及脑脊液中TNF-α、CRP含量下降,差异均显著(P<0.05)。结论:醒脑静联合血府逐瘀汤能有效缓解SAH大鼠CVS,与降低SAH大鼠血清及脑脊中TNF-α、CRP水平有关。Objective： To investigate effects of XingNaoJing injecta combining with XueFuZhuYudecoction on the expression levels of CRP and TNF-α in rats with subarachnoid hemorrhage rats with subarachnoid hemorrhage. Methods： 50 rats were divided into 5 groups randomly： normal group, model group, XingNaoJing group （0.3 mL/kg）, XueFuZhuYu decoction group （0.3 mL/kg） and the combining group （XingNaoJing inject combining with XueFuZhuYu decoction）. All SAH models of the groups except the normal group were established by the method of two times injections of arterial blood into cistema magna. The administration was started from the first day after the model establishment and lasted for 7 days. Equal physiological saline was given to the normal group and the model group. The serum and cerebrospinal fluid was extracted on 1 d, 3 d, 7 d, 14 d preoperatively and postoperatively. The content of tumor necrosis factor-ix （TNF-ct） and C-reaction protein was evaluated according to the ELISA kit instructions. Results： （1）After operation, the levels of D and TNF- in serum and cerebrospinal fluid of SAH rats increased gradually and reached the peak at seventh CRP. （2） On the 1 st postoperative day, compared with the normal group, model group in serum and cerebrospinal fluid （CSF） TNF - and CRP levels were significantly higher in, significant differences （P〈0.05）; compared with the model group, XingNaoJing group, Xuefu by Zhuyu decoction group in serum and cerebrospinal fluid （CSF） TNF-and CRP were no difference （P〉0.05）, and in the combination group in serum and cerebrospinal fluid （CSF） TNF - contentdecreased, thedifferenee is significant （P〈0.05）. （3） After the operation 3 days, 7 days, 14 days, compared with the normal group, model group in serum and cerebrospinal fluid （CSF） TNF-and CRP levels were significantly higher with significant differences （P〈0.05）; compared with the model group, XingNaoJing group, Xuefu by Xuefu Zhuyudecoction group and combination gr

关 键 词：醒脑静 血府逐瘀汤 蛛网膜下腔出血 肿瘤坏死因子-α C-反应蛋白 

分 类 号：Q953[生物学—动物学] R743.35[医药卫生—神经病学与精神病学]