不同年龄组人表皮干细胞Integrin β1表达分析研究  

Expression of β1-Integrin in Epidermal Stem Cells among Different Age Groups

作　　者：苏宁[1] 李新实[1] 胡晓蕊 朱建宇[1] 郑洪艳[1] 王昌涛[3] 杨丽[1] 霍彤[3] 赵丹[3] 

机构地区：[1]中国检验检疫科学研究院,北京100123 [2]北京华恒汉方制药有限公司,北京100082 [3]北京工商大学,北京100048

出　　处：《现代生物医学进展》2015年第33期6429-6431,6428,共4页Progress in Modern Biomedicine

基　　金：国家质检总局质检公益性行业科研专项项目(201310132;201410019)

摘　　要：目的:分析不同年龄组包皮组织中表皮干细胞Integrinβ1的表达,为组织工程学种子细胞的选用提供依据。方法:利用DispaseⅡ和BSA共同作用,分散表皮和真皮组织部分。对不同年龄组分离的表皮干细胞进行Integrinβ1免疫荧光染色,利用流式细胞仪检测不同年龄组Integrinβ1表达的阳性率。结果:不同年龄组分离得到的表皮干细胞形态一致。细胞生长状态和生长速度以7岁年龄组为最佳,其次是16岁年龄组,最后是30岁年龄组。流式细胞仪检测不同年龄组Integrinβ1的表达,7岁组阳性率为93%,16岁组阳性率为56%,30岁组阳性率仅为3.5%。结论:随着年龄的增长,皮肤组织中表皮干细胞的含量降低,低年龄段皮肤组织是组织工程学种子细胞的良好选择。Objective： To investigate the differential expression of Integrin β1 in epidermal stem cells among different age groups. Methods： The Epidermal stem cells were obtained by digesting human foreskin with Dispase II and BSA and Trypsin. The expression of Integrin β1 of epidermal stem cells was detected by Flow cytometry （FCM）. Results： The epidermal stem cells marked with β1-integrin and sorted by flow cytometry were similar in shape and size among different age groups. The cell growth state of the group aged 7were the best among the three groups. The rate of positive expression of β1-integrin in the group aged 7, aged 16 and aged 30 were 93%,56%, 3.5%, respectively. Conclusions： The expression of integrin β1 in three epidermal stem cells was decreased with the age, suggesting that the stem cells in skin tissues are decreased with age, and the lower age will be the best choice for tissue engineering.

关 键 词：表皮干细胞 分离 培养 INTEGRIN β1 流式细胞仪 

分 类 号：Q813.1[生物学—生物工程]