一起沙门菌引起的食源性疾病爆发的溯源分析  

Source Tracing of One Outbreak of Foodborne Disease Caused by Salmonella

作　　者：师舞阳[1] 郑悦康[1] 叶志英[1] 刘绮明 吴灿权[1] 

机构地区：[1]中山市疾病预防控制中心,广东中山528403

出　　处：《现代生物医学进展》2015年第31期6043-6045,6111,共4页Progress in Modern Biomedicine

基　　金：中山市社会发展公关计划(医疗)(20122A134)

摘　　要：目的:对一起沙门菌引起的食源性疾病爆发进行溯源分析。方法:采用GB4789法对采集的样品进行分离及鉴定,采用16S r RNA基因分型方法及PFGE分型方法对分离的菌株进行分子生物学分析,并对爆发进行溯源分析。结果:生化及血清学结果表明,该起爆发分离的菌型为伦敦沙门氏菌。16S r RNA基因分型表明爆发所分离的菌株均为肠道沙门菌肠道亚种,菌株12 sam与其他4个菌株分子发育距离较远,均为16S r RNA基因分型的TYPE1-11型;PFGE分型结果表明菌株10 sam、16 sam、27 sam及29sam的PFGE带型相似度为100%,菌株12sam跟其他菌株相似率为96%。结论:GB4789法结果表明该起爆发是由伦敦沙门氏菌引起的,16S r RNA基因分型及PFGE分型方法的结果均表明该起食源性疾病来源一致。Objective： To trace the source of one foodborne disease outbreak caused by salmonella using 16 S r RNA genotype method and pulsed-field gel electrophoresis. Methods： The strains（10 sam, 12 sam, 16 sam, 27 sam and 29 sam） from the outbreak were isolated and characterized by using GB4789 method for the culture, biochemical and serological characteristics. And two molecular typing methods were used for genotyping for the strains. Phylogenetic affiliation of the strains was studied using 16 S r RNA genotype and pulsed-field gel electrophoresis（PFGE） was used by characterizing Xba I to digest genomic DNA from Salmonella enteric. Results： The strains from this outbreak were identified as London Salmonella via culture, biochemical and serological results（GB4789）; they shared the same biochemical and serological characteristics. All the strains were closely associated with Salmonella enterica subsp. Enteric and classified into TYPE1-11 via 16 S r RNA genotyping, but the phylogenetic distance between strain 12 sam and the other four strains（10sam, 16 sam, 27 sam and 29 sam） was slightly further. While, The PFGE analysis revealed that the four strains（10 sam, 16 sam, 27 sam and 29 sam） shared the same banding pattern and the similarity between the strain 12 sam and the other four strains was 96 %. Conclusions： Using GB4789 and two typing approaches, we showed that this foodborne disease was caused by London Salmonella. 16 S r RNA genotype demonstrated excellent analytical sensitivity for tracing the source of the outbreak, and also PFGE has good discriminatory power and has proven highly reliable. Results from the present study suggest, 16 S r RNA genotype coupled with PFGE could be a useful molecular diagnostic method that could enhance the current diagnostic and surveillance methods of Salmonella infections.

关 键 词：食源性疾病 沙门菌 16S r RNA基因分型 PFGE 

分 类 号：R378.22[医药卫生—病原生物学] R117[医药卫生—基础医学]