AKR1B10与GPC-3免疫组化检测对肝细胞肝癌的诊断价值  

Application of AKR1B10 and GPC-3 in Diagnosis of Hepatocellular Carcinoma Immunohistochemistry

作　　者：温实[1] 戴新武[2] 黄丽娟[2] 张丽洁 李玉华[2] 

机构地区：[1]河北省保定市第一医院病理科,河北保定071000 [2]新疆生产建设兵团第二师库尔勒医院,新疆库尔勒841000 [3]海军北京丰台干休所卫生所,北京100071

出　　处：《现代生物医学进展》2015年第32期6263-6265,共3页Progress in Modern Biomedicine

基　　金：国家自然科学基金项目(30901795)

摘　　要：目的:探讨醛酮还原酶家族1B10(Aldo-keto reductase family 1,member B10,AKR1B10)与磷脂酰肌醇蛋白多糖-3(glypican-3,GPC-3)免疫组化检测在诊断肝细胞癌(HCC)的应用价值。方法:收集本院56例肝细胞癌,采用AKR1B10、GPC-3和联合法免疫组化染色,比较三种检测方法肝细胞癌的表达及强度。结果:三种免疫组化检测中,AKR1B10组、GPC-3组、联合组的阴性率分别为三组阳性率分别为73.21%、80.36%、91.07%,差异有统计学差异(P<0.05)。结论:AKR1B10与GPC-3免疫组化检测对HCC诊断均具有高度特异性、敏感性,AKR1B10与GPC-3联合法准确率和有效率更高,对肝癌患者预后有重大意义。Objective： To investigate the immunohistochemical detection of AKR1B10 and GPC-3 in the diagnosis of hepatocellular carcinoma. Methods： 56 patients with hepatocellular carcinoma who were diagnosed in our hospital were selected, and the AKR1B10 and GPC-3 were detected by immunohistochemical staining. Then the expressions and the intensity of hepatocellular carcinoma of the three detection methods were compared. Results： The negative rate ofAKR1B10, GPC-3 and combination was 73.21%, 80.36% and 91.07%, respectively. There was statistically significant difference between the three groups （P〈0.05）. Conclusion： AKR1B 10 and GPC-3 immunohistochemical detection are highly specific and sensitive to the diagnosis of HCC. AKR1B 10 and GPC-3 combination method has higher accuracy and efficiency, which is of great significance for the prognosis.

关 键 词：AKR1B10 GPC-3 肝细胞肝癌 免疫组化法 

分 类 号：R735.7[医药卫生—肿瘤]