基于石墨烯-纳米金修饰分子印迹传感器测定黑茶中芦丁  

Study on graphene-nanogold modified molecular imprinting electrochemical sensor for determining the content of rutin in black tea

作　　者：刘蓉[1] 雷存喜[1] 刘石泉[1] 周莉[1] 黄小柳[1] 

机构地区：[1]湖南城市学院化学与环境工程学院,益阳413000

出　　处：《分析试验室》2015年第11期1272-1275,共4页Chinese Journal of Analysis Laboratory

基　　金：湖南省教育厅高校科研一般项目(15C0244);湖南省科技计划项目(2012FJ3023);湖南省应用基础研究基地开放基金项目(湘科计字(2012)154号);湖南省自然科学基金项目(13JJ6074);湖南省科学技术厅国际合作项目(2012WK2013);湖南省高校科技创新团队支持计划资助项目(2014)207号);益阳市指导性科技计划项目(2014JZ26)资助

摘　　要：在金电极表面滴涂石墨烯（GR）,通过电沉积技术沉积纳米金（Au）构成石墨烯-纳米金修饰电极（Au-GR/GE）。以芦丁为模板分子,邻氨基酚为功能单体,通过电聚合反应在Au-GR/GE表面合成一种对芦丁具有特异性识别能力的分子印迹传感器膜（MIP）。采用循环伏安法（CV）、差分脉冲伏安法（DPV）研究了印迹膜的性能、结构和分子印迹效应,并与槲皮素进行了选择性响应的比较,发现此传感器对芦丁具有良好的选择性。在最佳实验条件下,其对芦丁浓度的定量测定线性范围为6.30×10^-7~1.70×10^-4mol/L,线性方程为I（μA）=3.8136-8.6247 lg c（mol/L）,R=0.9961,检出限为2.10×10^-7mol/L。The Au-GR/GE sensor was fabricated for detection of Rutin by drop coating of graphene（ GR） on GE（ gold electrode） and electrodeposition of nanogold paticles（ Au）. Using rutin as template molecule,oaminophenol as the functional monomer,the molecular imprinting polymer（ MIP） electrochemical sensor which had a specific recognition to rutin was formed on the surface of Au-GR/GE through polymerization reaction. In this study,the performance,structure and function of MIP were investigated by cyclic voltammetry（ CV） and differential pulse voltammetry（ DPV）. Quercetin which had a similar structure to rutin was used to compare the responses and the result showed that the sensor had a good selectivity of rutin. Under the optimized experimental conditions,the current response of the imprinted sensor was linear to the concentration of rutin in the range of6. 30 × 10^- 7~ 1. 70 × 10^- 4mol/L with linear equation as follows： I（ μA） = 3. 8136-8. 6247 lgc（ mol/L）,R =0. 9961,and a limit of detection was 2. 10 × 10^- 7mol/L. The sensor was used to determine the content of rutin in black tea samples,and the result was satisfactory.

关 键 词：芦丁 电沉积 石墨烯-纳米金复合材料 分子印迹 

分 类 号：TS272[农业科学—茶叶生产加工]