自体骨髓间充质干细胞治疗放射性骨坏死的动物实验  

Preliminary study on treatment of mandibular osteoradionecrosis by autologous bone marrow mesenchymal stem cells in miniature pigs

作　　者：房殿吉[1] 李松[2] 翟璐璐[3] 王占义[2] 郭延伟[1] 宁兆荣[1] 

机构地区：[1]佳木斯大学附属口腔医院口腔颌面外科,黑龙江154000 [2]长春市口腔医院口腔颌面外科 [3]吉林大学植物科学学院

出　　处：《中华口腔医学杂志》2015年第11期671-674,共4页Chinese Journal of Stomatology

基　　金：国家自然科学基金（81170935）

摘　　要：目的 探讨自体骨髓间充质干细胞（bone marrow mesenchymal stem cells,BMMSC）作为种子细胞复合羟基磷灰石（hydroxyapatite,HA）、磷酸三钙（tricalcium phosphate,TCP）治疗放射性骨坏死（mandibular osteoradionecrosis,ORN）的疗效.方法 6只小型猪分为实验组（4只）和对照组（2只）,对所有动物的下颌骨右侧一次性直线加速器照射25 Gy,建立ORN动物模型.分离培养小型猪BMMSC、实验组将扩增后的BMMSC复合至HA-TCP材料上,自体回植入已清创的ORN骨缺损部位,对照组仅植入HA-TCP.定期行大体观察、三维螺旋CT、冠状CT和组织病理学检查.结果 大体观察,实验组回植后3个月瘘管消失;对照组瘘管依然存在.三维螺旋CT观察,实验组回植后4个月骨皮质明显修复;对照组骨质破坏范围加大.冠状CT观察,实验组回植后4个月,骨皮质明显修复;对照组骨皮质及骨松质断裂,骨髓腔仍与外界相通.组织病理学检查,实验组回植4个月材料周围有新骨形成,骨陷窝内骨细胞清晰可见;对照组植入体内部大量新生纤维组织,未见新生骨组织.结论 BMMSC体外扩增后与HA-TCP复合植入ORN骨缺损部位具有治疗修复ORN的疗效.Objective To investigate the treatment effect of mandibular osteoradionecrosis（ORN） by autologous bone marrow mesenchymal stem cells（BMMSC） in miniature pigs.Methods Six miniature pigs with mandibular ORN（25 Gy） were used.BMMSC were separated and cultured in vitro and then implanted on to the premolded hydroxyapatite-tricalcium phosphate（HA-TCP）.The BMMSC-HA-TCP complexes were implanted into the defective area of mandibular ORN in 4 animals.Implantation of only HA-TCP in 2 animals served as control.Gross observation, spiral CT, coronary CT and histopathologic examination were carried out.Results Fistula disappeared in animals with BMMSC-HA-TCP complexes 3 months after implantation, while fistula was found in controls.Spiral CT analysis showed that cortical bone repair were found 4 months after BMMSC-HA-TCP complexes implantation, while bone damage（cortical and cancellous bone fracture） increased in controls.Histopathologic examination revealed that 4 months later after BMMSC-HA-TCP complexes implantation, new bone formation and bone cells could be observed, but there was a large number of fibrous tissue and no new bone in controls.Conclusions Transplantation of autologous BMMSC with HA-TCP may have therapeutic effect in the treatment of mandibular ORN.

关 键 词：骨坏死 放射性 骨髓祖代细胞 羟基磷灰石类 磷酸三钙 

分 类 号：R730.55[医药卫生—肿瘤]