Rapid Identification of Mycobacterium Leprae by Polymerase Chain Reaction-restriction Fragment Length Polymorphism Analysis of the Heat Shock Protein 65 Gene from Skin Specimens  

作　　者：Zheng Zhao Xi-Wan Liu Jun Jia Lin Cai Jian-Zhong Zhang 

机构地区：[1]Department of Dermatology, Peking University Peopte's Hospital, Beijing 100044, China

出　　处：《Chinese Medical Journal》2015年第21期2964-2966,共3页中华医学杂志（英文版）

基　　金：The study was supported by a grant from the National Natural Science Foundation of China （No. 81271761）.

摘　　要：INTRODUCTIONLeprosy caused by Mvcobacterium leprae （M. leprae）, is a chronic granulomatous disease affecting the skin and peripheral nervous system, which is transmitted through direct contact with nontreated or inadequate treatment patients. Diagnosis of leprosy depends on the clinical signs and symptoms and slit skin smear positivity. However, it＇s sometimes similar with other granulomatous disease caused by mycobacterial infection. Early stage leprosy is difficult to diagnose by clinical criterion alone because the sensitivity of acid-fast bacilli staining is quite low. The polymerase chain reaction-restriction fragment length polymorphism （PCR-RFLP） shows the great advantage in rapid identification and diagnosis for early cases and has a differentiation between leprosy and nonleprosy cases.INTRODUCTIONLeprosy caused by Mvcobacterium leprae （M. leprae）, is a chronic granulomatous disease affecting the skin and peripheral nervous system, which is transmitted through direct contact with nontreated or inadequate treatment patients. Diagnosis of leprosy depends on the clinical signs and symptoms and slit skin smear positivity. However, it＇s sometimes similar with other granulomatous disease caused by mycobacterial infection. Early stage leprosy is difficult to diagnose by clinical criterion alone because the sensitivity of acid-fast bacilli staining is quite low. The polymerase chain reaction-restriction fragment length polymorphism （PCR-RFLP） shows the great advantage in rapid identification and diagnosis for early cases and has a differentiation between leprosy and nonleprosy cases.

关 键 词：Heat Shock Protein 65 Gene Mycobacterium Leprae Polymerase Chain Reaction-restriction Fragment LengthPolymorphism 

分 类 号：Q78[生物学—分子生物学] X172[环境科学与工程—环境科学]