脑内IL-1β和ICAM-1与继发性脑损伤关系  

Relationship between Changes in Intracerebral IL-lp and ICAM-1 and the Secondary Brain Injury after Brain Injury

作　　者：朱刚[1] 王正国[2] 朱佩芳[2] 王宪荣[1] 

机构地区：[1]第三军医大学西南医院神经外科,重庆400038 [2]第三军医大学野战外科研究所,重庆400042

出　　处：《中国临床神经外科杂志》2002年第4期230-232,共3页Chinese Journal of Clinical Neurosurgery

基　　金：国家自然科学基金重点项目资助(No.39630330)

摘　　要：目的 探讨脑撞击伤后脑内白细胞介素-1β(IL-1β)和细胞间粘附分子-1(ICAM-1)变化及其介导的炎性损伤在继发性脑损伤(SBI)中的作用。方法 用落体撞击致大鼠一侧半球局部脑挫裂伤,用半定量酶联免疫吸附(ELISA)、生物化学及组织病理学方法观测脑创伤后脑组织IL-1β和ICAM-1含量、脑含水量、脑组织髓过氧化物酶(MPO)和超氧歧化酶(SOD)活性、脑组织丙二醛(MDA)含量变化,以及多形核粒细胞(PMN)侵入脑实质和脑实质的病理改变。结果 伤侧脑组织IL-1β和ICAM-1含量、脑含水量及MPO、MDA活性显著增加,SOD活性减小。PMN侵入并聚集于脑伤灶及其周围脑组织。结论 脑组织PMN聚集和MDA增加与脑创伤后IL-1β和ICAM-1含量增加有关。炎性损伤在SBI的发病过程中可能起重要作用。Objective To investigate the relationship between the of cerebral IL-1β and ICAM-1 contents and the secondary brain injury (SBI) after impacted cerebral trauma in the rats. Methods The cerebral contusion was induced by free-falling in the rats. IL-1β, ICAM-1 and water contents, activities of MPO and SOD and MDA infiltration of polymorphonudear (PMN) cells in the brain tissues, and cerebral pathological changes were measured respectively by the methods of ELISA, biochemistry and pathology. Results The contents of the cerebral IL-1β, ICAM-1 and water, and activity of MDA in the injured brain were significantly higher than that in the control brain (P<0.05). The significantly decrease in the activity of SOD of the injured brain was observed in the rats with in brain injury. There were many PMN cells in the injured brain tissue and one surrounding them. Conclusions The infiltration and accumulation of PMN cells and increase in MDA of the injured brain were related to increase in the intracerebral IL-1β and ICAM-1 in the rats with brain injury. The inflammatory injury may play an important role in SBI.

关 键 词：继发性脑损伤 白细胞介素-1Β 细胞间粘附分子-1 多形核粒细胞 SBI 脑水肿 

分 类 号：R742[医药卫生—神经病学与精神病学]