上调miRNA-100对胃癌SGC-7901细胞侵袭和转移的影响  

Influence of up-regulating miRNA-100 expression on invasion and metastasis of gastric cancer SGC-7901 cells in vitro

作　　者：李辉成[1] 刘素菊[2] 刘丽波[1] 徐振国[1] 贯海燕[1] 张凯[1] 

机构地区：[1]吉林大学第四医院血液肿瘤科,吉林长春130021 [2]吉林大学第四医院妇产科,吉林长春130021

出　　处：《中国临床研究》2015年第11期1422-1424,1428,共4页Chinese Journal of Clinical Research

摘　　要：目的探讨微小核糖核酸(miRNA)-100对人胃癌SGC-7901细胞侵袭、转移能力的影响。方法体外培养人胃癌SGC-7901细胞,将细胞分为空白对照组、阴性对照组和miRNA-100转染组。应用脂质体法,miRNA-100转染组、阴性对照组分别用构建的miRNA模拟物、阴性对照转染SGC-7901细胞。采用逆转录-聚合酶链反应(RTPCR)法检测各组细胞中miRNA-100的表达水平,细胞划痕实验检测细胞的迁移能力,Tranwell实验检测细胞的侵袭能力。结果 RT-PCR结果显示,miRNA-100转染组miRNA-100的表达量明显高于空白对照组和阴性对照组(P均<0.05)。细胞划痕实验显示,与空白对照组及阴性对照组比较,24 h和48 h时miRNA-100组周边细胞迁移至中央划痕区的距离明显增大,差异有统计学意义(P均<0.01)。Transwell实验显示,与空白对照组和阴性对照组相比,转染miRNA-100后,穿越小室的SGC-7901细胞数目明显减少,差异具有统计学意义(P<0.05)。结论转染miRNA-100可使SGC-7901细胞的迁移能力明显降低、细胞的侵袭能力被抑制,上调miRNA-100能抑制人胃癌SGC-7901细胞的侵袭、转移能力。Objective To investigate the effect of nficro ribonucleic acid （microRNA, miRNA）-100 expression on inva- sion and metastasis of human gastrie cancer SGC-7901 cells. Methods The SGC-7901 cells were cultured in vitro and di- vided into three groups： blank control group, negative control group and nliRNA-100 translection group. Using liposome method,the synthetic microRNA-100 mimic and negative control were respectively transfected into SGC-7901 cells in trans-tection group and negative control group. The expression levels of miRNA-100 in SGC-7901 cells in three groups were de- tected by reverse transcription- polymerase chain reaction（ RT-PCR）. The migratol of SGC-7901 cells was observed by cell wound scratch test. The invasion ability of SGC-7901 cells was measured by Transwell test. Results RT-PCR showed that miRNA-100 expression level in transfeetion group was significantly higher than that in blank control group and negative control group （ all P 〈 0.05 ）. The cell wound scratch test showed that the migration distances from periphral cell to central scratched area at 24h and 48h after transfection enlarged significantly in transfection group compared with blank control group and negative control group （ all P 〈 0.01 ）. Transwell test showed that the number of SGC-7901 cells passed through Transwell chamber in transfection group significantly decreased compared with blank control group and negative control group （ all P 〈 0.05 ）. Conclusions The translection of miRNA-100 can decline migratory ability and inhibit of invasion a- bilily of SGC-7901 cells. Up-regulation of miRNA-100 can suppress the metastasis and invasion of SGC-7901 cells.

关 键 词：微小核糖核酸 微小核糖核酸-100 胃癌 细胞划痕实验 Transwell实验 逆转录-聚合酶链反应 转移 侵袭 

分 类 号：R73-37[医药卫生—肿瘤]