一株酵母菌的鉴定及其挥发性物质防病测定  

Identification of a Yeast Strain and Effect of Its Volatile Organic Compounds on Disease Control

作　　者：黄蓉[1] 黄盼[2] 黄瑞荣[1] 李国庆[2] 

机构地区：[1]江西省农业科学院植物保护研究所,江西南昌330200 [2]华中农业大学湖北省作物病害监测与安全控制重点实验室,湖北武汉430070

出　　处：《江西农业大学学报》2015年第5期903-908,共6页Acta Agriculturae Universitatis Jiangxiensis

基　　金：国家自然科学基金青年科学基金项目(31201562);国家公益性行业(农业)科研专项(201303025);江西省农业科学院创新基金博士启动项目(2011CBS007)

摘　　要：为进一步研究用于防治草莓灰霉病的生防酵母菌,针对一株经平板测定和离体接种筛选得到的酵母菌菌株W4682,进行分类鉴定,并对其产生的挥发性物质防治草莓灰霉病的能力进行测定。结果发现,菌株W4682应属于汉逊德巴利酵母(Debaryomyces hansenii)。菌株W4682产生的挥发性物质不但可以抑制灰霉菌的生长,对草莓灰霉病的发生也具有显著的防治效果。同时菌株W4682接种草莓后不仅可以在被接种果实上定殖,控制其病害发生,其产生挥发性物质还可以控制同一密闭空间内未接种的草莓上病害的发生。因而,汉逊德巴利酵母菌株W4682具有较好的应用潜力。To verify the biocontrol efficacy of antagonistic yeast fungus in control of strawberry gray mold disease,a yeast strain W4682 which was effective in inhibiting Botrytis cinerea in dual culture and suppressing gray mold disease on strawberry fruits with the live cells,was identified and the efficacy of its volatile organic compounds in control of strawberry gray mould was determined. As a result,the yeast strain W4682 was identified as Debaryomyces hansenii. The volatile organic compounds produced by the strain W4682 could inhibit mycelial growth of Botrytis cinerea and suppress botrytis fruit rot in strawberry obviously. When the strain W4682 was inoculated on strawberry fruits,the yeast cells colonized on fruit surface and controlled the botrytis rot in these fruits effectively,at the same time,the volatile organic compounds produced by these fruits also effectively controlled the botrytis rot in other fruits under the same air-tight space which were not inoculated with yeast cells. This study suggests that the strain W4682 of Debaryomyces hansenii is a promising agent for the control of Botrytis cinerea.

关 键 词：DEBARYOMYCES hansenii 挥发性物质 灰霉病 

分 类 号：S476.8[农业科学—农业昆虫与害虫防治]