赤芝子实体的化学成分及其抗肿瘤活性  

Chemical constituents from fruiting body of Ganoderma lucidum and their antitumor activity

作　　者：熊小文[1] 李晔 李鹏[1,3] 姚广[4] 张志强[1] 许建华[1,3] 

机构地区：[1]福建医科大学药学院,福建福州350108 [2]福建仙芝楼生物科技有限公司,福建福州350003 [3]福建医科大学福建省高校天然药物药理学重点实验室,福建福州350108 [4]美国亚利桑那大学分子细胞生物学系,美国亚利桑那州图森85721

出　　处：《中国医院药学杂志》2015年第21期1902-1906,共5页Chinese Journal of Hospital Pharmacy

基　　金：国家自然科学青年科学基金(编号:81202560);福建省自然科学基金(编号:2015J01678);国家国际科技合作专项(编号:2013DFA30900)

摘　　要：目的：研究赤芝子实体的化学成分及其体外抗肿瘤活性。方法：用硅胶柱色谱法,Sephadex LH-20柱色谱法及制备型高效液相色谱法进行分离纯化,依据理化性质和光谱数据鉴定化合物结构;用MTT法测试化合物体外抗肿瘤活性。结果：分离得到6个化合物,其结构鉴定为ganoderone A（1）、麦角甾醇（2）、麦角甾醇过氧化物（3）、麦角甾-7、22-二烯-3β,5α,6β-三醇（4）、灵芝酸DM（5）和7-oxo-灵芝酸Z（6）;化合物1,3对人慢性粒细胞白血病细胞K562和人结肠癌细胞SW620的IC50分别为9.61,10.70 mg·L-1和22.38,32.48 mg·L-1,化合物1~6对人急性髓系白血病细胞HL60的IC50分别为7.14,11.07,2.80,7.54,51.82和7.39 mg·L-1。结论：ganoderone A为首次从赤芝中分离得到;灵芝中的甾醇及三萜类化合物均有一定抗肿瘤活性。OBJECTIVE To study on chemical constituents from fruiting body of Ganoderma lucidum and their antitumor activity in vitro. METHODS Chemical constituents were isolated and purified by silica gel,Sephadex LH-20 column chromatography and preparative high performance liquid chromatography. The structures were identified by means of physico-chemical property and spectral data. Antitumor activity of the compounds were screened by using standard MTT method in vitro. RESULTS Six constituents were isolated from fruiting body of G. lucidum,and their structures were elucidated as ganoderone A（ 1）,ergosterol（ 2）,ergosterol peroxide（ 3）,ergosta-7,22-dien-3β,5α,6β-triol（ 4）,ganoderic acid DM（ 5） and 7-oxo-ganoderic acid Z（ 6）. The 50% inhibiting concentration（ IC50） of compounds 1,3 for cancer cell lines K562 and SW620 were 9. 61,10. 70 mg·L- 1and 22. 38,32. 48 mg·L- 1,respectively. The IC50 of compounds 1-6 for cancer cell line HL60 were 7. 14,11. 07,2. 80,7. 54,51. 82 and 7. 39 mg·L- 1,respectively. CONCLUSION Compound 1 is isolated from G. lucidum for the first time. Ganoderma sterol and triterpene have certain antitumor activities.

关 键 词：灵芝 化学成分 抗肿瘤 

分 类 号：R914[医药卫生—药物化学]