胰腺癌淋巴结转移小鼠外周血免疫细胞群的变化  

The changes of immune cell populations in peripheral blood of mice with lymph node metastasis of pancreatic cancer

作　　者：刘乔飞[1] 牛哲禹[1] 李媛[2] 王梦一[1] 卢朝辉[2] 廖泉[1] 赵玉沛[1] 

机构地区：[1]中国医学科学院北京协和医院基本外科,北京100730 [2]中国医学科学院北京协和医院病理科,北京100730

出　　处：《基础医学与临床》2015年第11期1471-1475,共5页Basic and Clinical Medicine

基　　金：国家自然科学基金(81272573)

摘　　要：目的观察胰腺癌淋巴结转移小鼠外周血免疫细胞群的变化。方法足底注射C57BL/6J小鼠同源Panc02胰腺癌细胞,流式细胞计数检测外周血中13个免疫细胞群的变化。结果 6周后有淋巴结转移的转移率为65%。与正常对照小鼠相比,非淋巴结转移及淋巴结转移小鼠外周血中B淋巴细胞(P<0.05,P<0.01)、T淋巴细胞(P<0.05,P<0.01)均明显减少;而粒细胞(P<0.05,P<0.01)、髓系来源抑制细胞(MDSC)(P<0.01,P<0.01)以及M2型肿瘤相关巨噬细胞(TAM)(P<0.05,P<0.01)均明显增多。淋巴结转移小鼠较非淋巴结转移小鼠上述变化更明显(P<0.05)。结论胰腺癌淋巴结转移可引起强烈的免疫抑制效应,其中MDSC以及M2型TAM可能起到关键作用。免疫环境的重建可能是胰腺癌淋巴结转移防治的重要靶点。Objective To observe the changes of immune cell populations in peripheral blood of mice with lymph node metastasis of pancreatic cancer. Methods C57 BL /6J mouse syngeneic Panc02 pancreatic cancer cells were injected into intra-footpad. The FCM（ fluorescence activated cytometry） was adopted to detect 13 immune cell populations in peripheral blood. Results Six weeks after injection,the lymph node metastasis rate was 65%. Compared to that of normal mice,in the peripheral blood of the mice with and without lymph node metastasis,the B lymphocytes（ P 〈0. 05,P 〈0. 01） and T lymphocytes（ P 〈0. 05,P 〈0. 01） were significantly decreased; however,the granulocytes（ P 〈0. 05,P 〈0. 01）,myeloid derived suppressor cells（ MDSC）（ P 〈0. 01,P 〈0. 01） and M2 polarized tumor associated macrophages（ TAM）（ P 〈0. 05,P 〈0. 01） were significantly elevated. Compared with the tumor bearing mice without lymph node metastasis,in the peripheral blood of the tumor bearing mice with lymph node metastasis,the changes of the above immuen cell populations were more obvious（ P 〈0. 05）. Conclusions Pancreatic cancer with lmyph node metastasis can induce intensively systemic immunosuppression and the MDSC and M2 polarized TAM may play crucial roles. Immune reconstructions are potential important targets for the prevention and treatment of lymph node metastasis of pancreatic cancer.

关 键 词：胰腺癌 淋巴结转移 髓系来源抑制细胞 肿瘤相关巨噬细胞 免疫重建 

分 类 号：R735.9[医药卫生—肿瘤] R657.5[医药卫生—临床医学]